1 - Learning to Pipette worksheet_SP24

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Northeastern University *

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Course

1011

Subject

Chemistry

Date

Feb 20, 2024

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docx

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2

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Name :___ __ _________________________________ Lab group:___ _ ________________________________ _____________________________________ Post-Lab Worksheet: Learning to Pipette 20 pts.; due the week of January 29. This worksheet is to be completed and submitted individually. Follow your lab instructor’s directions regarding how and when to turn in your worksheet. 1. What common errors in handling a micropipettor can account for pipetting too much reagent into a tube? (5 pts.) The inability to read and adjust the measurement device accurately is common error made when using a micropipettor. Your micropipette quantity will be off if the micropipettor is set to the wrong measurement. The transfer on the outside of the micropipette tip may also require the addition of additional tube. This would however be a very modest amount of solution. 2. What common errors in handling a micropipettor can account for underpipetting reagent into a tube? (5 pts.) Reagent transfer to the tube may be reduced if the micropipettor is not adjusted correctly. Reagent would also be delivered into the tub in smaller amounts if there were air bubbles in the micropetting tip or if the stopper was pressed all the way before you wanted to release all of the liquid 3. Complete the following conversions: (2 pts. each) Be aware of the units! 1µl = ____ 0.001 ______ ml 750µl = ____ .75 ______ml 0.5L = ____ 500______ ml _____ 600 ____ µl = 0.6 ml Post-Lab Worksheet: Learning to Pipette Page | 1
___ 200______ ml = 0.2L Post-Lab Worksheet: Learning to Pipette Page | 2
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