Table #2 Worktable for BCA Assay Standard Water (μl) Tube # 1 2 3 4 5 6 7 8 9 0 125 325 175 325 325 325 400 400 BSA source (μl) 300 stock 375 stock 325 stock 175 of tube #2 325 of tube #3 325 of tube #5 325 of tube #6 100 of tube #7 0 Protein (ug) Add the volumes listed in Table 2 to each tube. Add the water first and then the appropriate standard. Pipette each of the protein samples directly into the water, do not dribble on the side of the test tubes. The standard is at a concentration of 2 mg/ml and contains bovine serum albumin (BSA). Calculate the amount of BSA protein added to each of the tubes and write this in Table 2 (Protein (ug)).
Table #2 Worktable for BCA Assay Standard Water (μl) Tube # 1 2 3 4 5 6 7 8 9 0 125 325 175 325 325 325 400 400 BSA source (μl) 300 stock 375 stock 325 stock 175 of tube #2 325 of tube #3 325 of tube #5 325 of tube #6 100 of tube #7 0 Protein (ug) Add the volumes listed in Table 2 to each tube. Add the water first and then the appropriate standard. Pipette each of the protein samples directly into the water, do not dribble on the side of the test tubes. The standard is at a concentration of 2 mg/ml and contains bovine serum albumin (BSA). Calculate the amount of BSA protein added to each of the tubes and write this in Table 2 (Protein (ug)).
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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![Table #2 Worktable for BCA Assay
Standard
Water (μl)
Tube #
1
2
3
4
5
6
7
8
9
0
125
325
175
325
325
325
400
400
BSA source (μl)
300 stock
375 stock
325 stock
175 of tube #2
325 of tube #3
325 of tube #5
325 of tube #6
100 of tube #7
0
Protein (ug)
Add the volumes listed in Table 2 to each tube. Add the water first and then the
appropriate standard. Pipette each of the protein samples directly into the water, do not
dribble on the side of the test tubes. The standard is at a concentration of 2 mg/ml and
contains bovine serum albumin (BSA). Calculate the amount of BSA protein added to each
of the tubes and write this in Table 2 (Protein (ug)).](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc02bfde4-a78d-4551-93f6-03d589a5c8dc%2F35aec282-c08a-40b8-8be4-9b9bd9ee209d%2Fzlinmi5_processed.png&w=3840&q=75)
Transcribed Image Text:Table #2 Worktable for BCA Assay
Standard
Water (μl)
Tube #
1
2
3
4
5
6
7
8
9
0
125
325
175
325
325
325
400
400
BSA source (μl)
300 stock
375 stock
325 stock
175 of tube #2
325 of tube #3
325 of tube #5
325 of tube #6
100 of tube #7
0
Protein (ug)
Add the volumes listed in Table 2 to each tube. Add the water first and then the
appropriate standard. Pipette each of the protein samples directly into the water, do not
dribble on the side of the test tubes. The standard is at a concentration of 2 mg/ml and
contains bovine serum albumin (BSA). Calculate the amount of BSA protein added to each
of the tubes and write this in Table 2 (Protein (ug)).
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