Experiment 9 Report Forms gg

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School

Mohawk College *

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Course

ENVR CY601

Subject

Chemistry

Date

Feb 20, 2024

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pdf

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2

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CHEM 10054 Experiment 9 Page 1 of 2 Experiment 9 Report Forms Name Student ID Name manisha Student ID 000832490 At the start of the lab, show your completed pre-lab calculations to your instructor and obtain the instructors initials. Instructor Initials: _______RM_________ Pre-Lab Calculations Calculate the concentration of caffeine prepared from a 1000 ppm caffeine stock solution if the following aliquots are diluted into a 10.00 mL volumetric flask. Table 1. Dilutions for the preparation of caffeine standards. Stock Caffeine C 1 (ppm) Aliquot Vol. of Stock V 1 ( L, microliter) Total volume V 2 (mL) Caffeine Conc. C 2 (ppm) 1 1000 100 10.00 10 2 1000 250 10.00 25 3 1000 350 10.00 35 4 1000 500 10.00 50 If a 500 L aliquot of your sample is diluted to 10.00 mL, the dilution factor is…… Dilution factor=_20_ Results Part A: Calibration of a micropipette Table 2. Calibration of the micropipette using water. Assume the density of water is 1.0000 g/mL. (perform at least 1 micro pipette check at each volume ) Target Volume ( L) Mass 1 (g) Mass 2 (g) Mass 3 (g) Average Volume ( L, microliter) 100 0.1012 0.1003 0.1005 100 250 0.2501 0.2502 0.2501 250 350 0.3500 0.3501 0.3500 350 500 0.5002 0.5003 0.5002 500 1000 1.005 1.007 1.004 1000 Question 1: Based on your results, is the micropipette calibrated? If not, then would it lead to incorrect/inaccurate results? Yes, the micropipette was calibrated because the average volume pipetted from the micropipette is almost same as the target volume.
CHEM 10054 Experiment 9 Page 2 of 2 Part B: Preparation and Analysis of Standard Solutions and Samples Enter the concentration, retention time and peak area for the samples of theophylline (flask 1), caffeine (flask 2) and adipic acid (flask 3). Table 3. Summary table for HPLC data Flask Compound Target Conc. (ppm) Exact Conc. (ppm) Retention Time (min) Peak Area (mAu s) 1 caffeine 10.0 10.0 7.267 161.259 2 theophylline 10.0 10.0 5.183 169.009 3 adipic acid 10.0 10.0 Not detected Not detected Note: 1. Please don’t panic if there are no peaks for flask 3 (adipic acid) or the peak is very small. Part C: Inject Samples in the HPLC Enter the caffeine concentration of the standards and report caffeine and theophylline, and peak areas. Table 4. HPLC data for calibration caffeine standards and the unknown sample. Flask # Target Caffeine Conc (ppm) Exact Caffeine Conc (ppm) Area Caffeine Peak (mAu s) Area Theophylline Peak (mAu s) Area Ratio A caf /A theo 4 10 10 208.509 400.242 0.5210 5 25 25 370.152 409.901 0.9057 6 35 35 491.817 407.054 1.2082 7 50 50 686.899 400.242 1.7162 8 unknown 371.252 266.652 1.3923
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