Microbiology: A Systems Approach
Microbiology: A Systems Approach
4th Edition
ISBN: 9780073402437
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Chapter 9.5, Problem 19AYP
Summary Introduction

To define:

The term mutation and also give one positive and negative example of it in microorganisms.

Introduction:

Mutation in an organism is defined as a sudden alteration of “nucleotide sequences”. The mutations can be harmful, beneficial, or neutral; depending on the location of alteration in the genome and the effect exerted by the environment.

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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?   2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?   3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol   -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes.   Questions: 1) What differences would you expect to see between the…
Overview of Transformation Protocol   -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes.   Questions: 1)What is the selectable marker in this experiment? How…

Chapter 9 Solutions

Microbiology: A Systems Approach

Ch. 9.2 - Identify the locations of the promoter, the start...Ch. 9.2 - Prob. 12AYPCh. 9.2 - Prob. 13AYPCh. 9.3 - Define the term operon and explain one advantage...Ch. 9.3 - Differentiate between repressive and inducible...Ch. 9.3 - Prob. 16AYPCh. 9.4 - Explain the defining characteristics of a...Ch. 9.4 - Describe three forms of horizontal gene transfer...Ch. 9.5 - Prob. 19AYPCh. 9.5 - Prob. 20AYPCh. 9 - Prob. 1CFCh. 9 - What is the smallest unit of heredity? a....Ch. 9 - The nitrogen bases in DNA are bonded to the a....Ch. 9 - DNA replication is semiconservative because the...Ch. 9 - In DNA, adenine is the complementary base for...Ch. 9 - Transfer RNA is the molecule that a. contributes...Ch. 9 - Prob. 6MCQCh. 9 - The lac operon is usually in the _____ position...Ch. 9 - Prob. 8MCQCh. 9 - Prob. 9MCQCh. 9 - When genes are turned on differently under...Ch. 9 - The DNA pairs are held together primarily by...Ch. 9 - Prob. 12TFCh. 9 - Prob. 13TFCh. 9 - Prob. 14TFCh. 9 - Prob. 15TFCh. 9 - Explain the relationship among the following...Ch. 9 - Prob. 2CTQCh. 9 - On paper, replicate the following segment of DNA:...Ch. 9 - Provide evidence in support of or refuting the...Ch. 9 - Prob. 5CTQCh. 9 - Prob. 6CTQCh. 9 - Prob. 7CTQCh. 9 - Prob. 8CTQCh. 9 - Prob. 9CTQCh. 9 - Use your knowledge of DNA recombination events to...Ch. 9 - Prob. 1CCCh. 9 - Prob. 2CCCh. 9 - Prob. 3CCCh. 9 - Prob. 1VCCh. 9 - From chapter 4, figure 4.11a. Speculate on why...Ch. 9 - Concept Mapping | Blooms Level 6: Create Appendix...
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Mitochondrial mutations; Author: Useful Genetics;https://www.youtube.com/watch?v=GvgXe-3RJeU;License: CC-BY