Microbiology: A Systems Approach
4th Edition
ISBN: 9780073402437
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 9, Problem 10CTQ
Use your knowledge of DNA recombination events to complete the following:
- a. Propose two ways in which antibiotic resistance may develop in a bacterium.
- b. Explain how transposable elements may be used to treat humans with mutations in insulin-producing genes.
- c. Describe how bacterial cells acquire the ability to produce toxins.
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In a process of production of a recombinant protein by E. coli cells, it was observed accumulation of acetate in the culture medium. In this situation, it can be said that:
(a) certainly the process in question was being conducted in anaerobiosis
(B).Acetate accumulation is advantageous for the process as the acetate formation reaction generates 1 molecule of ATP
(c)Knowing that decreasing the temperature of the process causes a reduction in the rate of glycolysis, this could be a strategy to reduce the accumulation of acetate
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A research group is studying a bacterium X that binds to mucosal cells in the lung and invades. Wildtype X has an LD50 value of 10 bacteria when administered to mice by inhalation. Using transposon mutagenesis, the researchers have isolated two mutants of X that they call Xmut1 and Xmut2, both of which have LD50 values of 105 when inhaled by mice. However, in tissue culture cells, Xmut1 can invade the cells just as well as wild-type X, while Xmut2 cannot. Provide a possible explanation for these results.
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a) For each of these drugs, identify at what point it could affect the process of DNA->RNA->protein. Be as specific as possible.
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Chapter 9 Solutions
Microbiology: A Systems Approach
Ch. 9.1 - Define the terms genome and gene.Ch. 9.1 - Differentiate between genotype and phenotype.Ch. 9.1 - Diagram a segment of DNA, labeling all important...Ch. 9.1 - Summarize the steps of bacterial DNA replication...Ch. 9.1 - Compare and contrast the synthesis of leading and...Ch. 9.2 - Explain how the classical view of the central...Ch. 9.2 - Prob. 7AYPCh. 9.2 - Illustrate the steps of transcription, noting the...Ch. 9.2 - Prob. 9AYPCh. 9.2 - Prob. 10AYP
Ch. 9.2 - Identify the locations of the promoter, the start...Ch. 9.2 - Prob. 12AYPCh. 9.2 - Prob. 13AYPCh. 9.3 - Define the term operon and explain one advantage...Ch. 9.3 - Differentiate between repressive and inducible...Ch. 9.3 - Prob. 16AYPCh. 9.4 - Explain the defining characteristics of a...Ch. 9.4 - Describe three forms of horizontal gene transfer...Ch. 9.5 - Prob. 19AYPCh. 9.5 - Prob. 20AYPCh. 9 - Prob. 1CFCh. 9 - What is the smallest unit of heredity? a....Ch. 9 - The nitrogen bases in DNA are bonded to the a....Ch. 9 - DNA replication is semiconservative because the...Ch. 9 - In DNA, adenine is the complementary base for...Ch. 9 - Transfer RNA is the molecule that a. contributes...Ch. 9 - Prob. 6MCQCh. 9 - The lac operon is usually in the _____ position...Ch. 9 - Prob. 8MCQCh. 9 - Prob. 9MCQCh. 9 - When genes are turned on differently under...Ch. 9 - The DNA pairs are held together primarily by...Ch. 9 - Prob. 12TFCh. 9 - Prob. 13TFCh. 9 - Prob. 14TFCh. 9 - Prob. 15TFCh. 9 - Explain the relationship among the following...Ch. 9 - Prob. 2CTQCh. 9 - On paper, replicate the following segment of DNA:...Ch. 9 - Provide evidence in support of or refuting the...Ch. 9 - Prob. 5CTQCh. 9 - Prob. 6CTQCh. 9 - Prob. 7CTQCh. 9 - Prob. 8CTQCh. 9 - Prob. 9CTQCh. 9 - Use your knowledge of DNA recombination events to...Ch. 9 - Prob. 1CCCh. 9 - Prob. 2CCCh. 9 - Prob. 3CCCh. 9 - Prob. 1VCCh. 9 - From chapter 4, figure 4.11a. Speculate on why...Ch. 9 - Concept Mapping | Blooms Level 6: Create Appendix...
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