Microbiology: A Systems Approach
4th Edition
ISBN: 9780073402437
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Chapter 9, Problem 13TF
Summary Introduction
Introduction:
The
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What proteins are crucial for creating and maintaining DNA replication forks? Choose the best explanation.
Question 2 options:
Helicase creates the replication fork; primase keeps the single strands from closing shut.
Helicase creates the replication fork; single-strand binding proteins keep the single strands from reuniting.
Ligase creates the replication fork; DNA polymerase II keeps the single strands from reuniting.
Helicase creates the replication fork; ligase keeps the single strands from closing shut.
Identify if the statement is correct or incorrect, "DNA ligase separates the two strands of the relaxed DNA helix, the point of separation is the replication fork."
DNA polymerases are processive, which means that they remain tightly associated with the
template strand while moving rapidly and adding nucleotides to the growing daughter
stand. Which piece of the replication machinery accounts for this characteristic?
Helicase
Sliding Clamp
Single Stranded Binding Protein
Primase
Chapter 9 Solutions
Microbiology: A Systems Approach
Ch. 9.1 - Define the terms genome and gene.Ch. 9.1 - Differentiate between genotype and phenotype.Ch. 9.1 - Diagram a segment of DNA, labeling all important...Ch. 9.1 - Summarize the steps of bacterial DNA replication...Ch. 9.1 - Compare and contrast the synthesis of leading and...Ch. 9.2 - Explain how the classical view of the central...Ch. 9.2 - Prob. 7AYPCh. 9.2 - Illustrate the steps of transcription, noting the...Ch. 9.2 - Prob. 9AYPCh. 9.2 - Prob. 10AYP
Ch. 9.2 - Identify the locations of the promoter, the start...Ch. 9.2 - Prob. 12AYPCh. 9.2 - Prob. 13AYPCh. 9.3 - Define the term operon and explain one advantage...Ch. 9.3 - Differentiate between repressive and inducible...Ch. 9.3 - Prob. 16AYPCh. 9.4 - Explain the defining characteristics of a...Ch. 9.4 - Describe three forms of horizontal gene transfer...Ch. 9.5 - Prob. 19AYPCh. 9.5 - Prob. 20AYPCh. 9 - Prob. 1CFCh. 9 - What is the smallest unit of heredity? a....Ch. 9 - The nitrogen bases in DNA are bonded to the a....Ch. 9 - DNA replication is semiconservative because the...Ch. 9 - In DNA, adenine is the complementary base for...Ch. 9 - Transfer RNA is the molecule that a. contributes...Ch. 9 - Prob. 6MCQCh. 9 - The lac operon is usually in the _____ position...Ch. 9 - Prob. 8MCQCh. 9 - Prob. 9MCQCh. 9 - When genes are turned on differently under...Ch. 9 - The DNA pairs are held together primarily by...Ch. 9 - Prob. 12TFCh. 9 - Prob. 13TFCh. 9 - Prob. 14TFCh. 9 - Prob. 15TFCh. 9 - Explain the relationship among the following...Ch. 9 - Prob. 2CTQCh. 9 - On paper, replicate the following segment of DNA:...Ch. 9 - Provide evidence in support of or refuting the...Ch. 9 - Prob. 5CTQCh. 9 - Prob. 6CTQCh. 9 - Prob. 7CTQCh. 9 - Prob. 8CTQCh. 9 - Prob. 9CTQCh. 9 - Use your knowledge of DNA recombination events to...Ch. 9 - Prob. 1CCCh. 9 - Prob. 2CCCh. 9 - Prob. 3CCCh. 9 - Prob. 1VCCh. 9 - From chapter 4, figure 4.11a. Speculate on why...Ch. 9 - Concept Mapping | Blooms Level 6: Create Appendix...
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- Describe the role of the proteins involved with DNA replication. Also identify if the protein is involved the leading strand, lagging strand or both. DNA Ligase DNA Polymerase I DNA Polymerase III Helicase Primase Single-stranded DNA-binding proteins Topoisomerasearrow_forwardWhich enzymes are involved in DNA replications? There are multiple answers. Helicase DNA polymerase RNA polymerase DNA ligase single strand binding proteins peptidyl transferase σ unit release factorsarrow_forwardDNA polymerase is important in the replication process because it can join together segments of DNA into a single daughter strand. Select one: True Falsearrow_forward
- Okazaki fragments are short DNA pieces that explain how the DNA polymerase can continue the synthesis of the new strand. True Falsearrow_forwardDuring the process of Initiation in DNA Replication, if one of the initiator proteins is not present, give specific details on what will happen.arrow_forwardReplication of nuclear DNA begins at the Ori (origin of replication). True or falsearrow_forward
- Which of the followings statements are true about DNA polymerase? 1.) It can only go in one direction, meaning the lagging strand can't be synthesized continuously. 2.) It cannot start a DNA strand from scratch, so another enzyme is needed to create "primers" as a starting point. 3.) It cannot copy epigenetic marks (such as methyl groups) on its own; these must be "copied" onto the daughter DNA strand by other enzymes after DNA replication. 4.) All of the abovearrow_forwardPlace the following steps of DNA replication in order (from left to right) from the beginning to the end of the process. Reset Help priming of DNA synthesis with RNA primer removal of primers by DNA polymerase I exonuclease elongation of RNA primers by DNA polymerase III opening and stabilizing the DNA strands joining of Okazaki fragments by DNA ligase Beginning of replication End of replicationarrow_forwardWhich of the following enzymes can break, and rejoin, phosphodiester bonds during the normal DNA replication process in the chromosomes of E. coli cells? single-stranded binding proteins RNA polymerase topoisomerase DNA helicase DNA ligasearrow_forward
- The enzyme required to open up the double stranded DNA during replication andform the replication fork (two single stranded DNA) is called aarrow_forwardThe figure below depicts various elements of the eukaryotic replication machinery in action. Enter the name for the protein depicted by each box. Box A Box B Box C Box D Box E Box F DNA polymerase on lagging strand (just finishing an Okazaki fragment) F Maintains polymerase association with DNA Enzyme extends separation of DNA strands Synthesizes RNA fragments that hybridize to DNA Relaxes supercoiled DNA ahead of replication fork Maintains DNA is single stranded state Promotes binding of processivity factors to DNA Newly synthesized strand pocoar Leading-strand template A New Okazaki fragment RNA primer E Lagging-strand template DNA polymerase on leading strand B C D Saaragon - Next Okazaki fragment will start here Parental DNA helixarrow_forwardFor each round of DNA replication, pokaryotic DNA starts replicating at:arrow_forward
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