Chapter 9, Problem 1AQ

Apart from genome size, what factors make complete assembly of a eukaryotic genome more difficult than assembly of a genome from a species of Bacteria or Archaea?

Summary Introduction

To explain:

What are the factors that make entire assembly of a eukaryotic genome is more difficult than the assembly of a genome from a species of Archaea and Bacteria.

Concept introduction:

The number of base pairs constitutes an organisms, genetic constitution is referred as the genome size. Using comparative genomics, it is determined that the Archaea and Bacteria genome reveals the strong correlation between the open reading frame (ORFs) and genome size. Despite of an organism, every DNA mega base pair in the cells of prokaryotes encodes about 1000 open reading frame (ORFs) and genome size increases, then the number of genes also increases proportionally.

Explanation of Solution

An essential challenge of acquiring the entire eukaryotic genome sequence includes that it has a multiple number of chromosome that frequently contains an extensive run of repetitive DNA, the genes are generally consists of introns that can constitute an increased percentage of the genome than compared to the exons and they continuously have a prolonged number of non-coding RNAs. It contains organelles and sometimes it also contains nucleomorphs that their possess genome.