EBK BROCK BIOLOGY OF MICROORGANISMS
EBK BROCK BIOLOGY OF MICROORGANISMS
15th Edition
ISBN: 8220103633352
Author: Stahl
Publisher: PEARSON
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Chapter 9.2, Problem 2MQ
Summary Introduction

Before analyzing or after the genome sequence is fragmented, the existing genome must be assembled. The assembly of the genome involves eliminating the overlaps and placing the fragments in the accurate arrangement. The genome assemble must be annotated and it can be helpful in determining the more functional regions and genes. The initial step in the annotation is to identify for the open reading frame (ORFs).

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What are “forcefields” and how are they used in predicting how a protein sequence will fold?
Using a table that shows which codon represents which amino acid determine the following:   A) The possible codons that encode Serine:    B) The amino acids that could be encoded if the 2nd position of the UCA codon that encodes Serine was changed to one of the other 3 bases:    C) The amino acids that could be encoded if the 3rd position of the UCA codon that encodes Serine was changed to one of the other 3 bases:    D) The amino acids that could be encoded if the 1st position of the UCA codon that encodes Serine was changed to one of the other 3 bases:
Suppose that there is an unknown protein that underwent Edman sequencing method. From N- terminal determination, a biochemist found out that there are two N-terminal amino acid residues, V and G. What is the original sequence of the protein given the following peptide fragments: after digestion with Chymotrypsin: G-L-S-R-G-M-w V-A-L-F Q-L-Y L-R-V-W G-M-V-E-A-D-I-P K-S-P-E-M-T-W R-M-A-S-E-K-P-G-H after digestion with Trypsin: P-G-H V-W-G-M-V-E-A-D-I-P M-A-S-E-K G-M-W-Q-L-Y-L-R S-P-E-M-T-W-R G-L-S-R V-A-L-F-K after digestion with Cyanogen Bromide: T-W-R-M W-Q-L-Y-L-R-V-W-G-M V-E-A-D-I-P A-S-E-K-P-G-H V-A-L-F-K-S-P-E-M G-L-S-R-G-M

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EBK BROCK BIOLOGY OF MICROORGANISMS

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