Whether Reaction 1 at equilibrium or Reaction 2 not at equilibrium does any work or not. Introduction: The equilibrium constant represents the relation between reactants and products in a reaction at equilibrium. The concentrations of reactants and products are constant at equilibrium. Under conditions of a biochemical reaction, free energy (G) is defined as the amount of energy available to perform work.

Question

1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results? 2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab? 3) Why are the cells incubated at 42°C?

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Chapter 7.3, Problem 2C

Summary Introduction

To determine: Whether Reaction 1 at equilibrium or Reaction 2 not at equilibrium does any work or not.

Introduction: The equilibrium constant represents the relation between reactants and products in a reaction at equilibrium. The concentrations of reactants and products are constant at equilibrium. Under conditions of a biochemical reaction, free energy (G) is defined as the amount of energy available to perform work.

Summary Introduction

To determine: Which reaction does work, Reaction 1 which is at equilibrium or Reaction 2 which is not at equilibrium.

Introduction: The equilibrium constant represents the relation between reactants and products in a reaction at equilibrium. The concentrations of reactants and products are constant at equilibrium. Under conditions of a biochemical reaction, free energy (G) is defined as the amount of energy available to perform work.

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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results? 2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab? 3) Why are the cells incubated at 42°C?

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…