Biology (MindTap Course List)
Biology (MindTap Course List)
10th Edition
ISBN: 9781285423586
Author: Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher: Cengage Learning
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Chapter 7.1, Problem 1C

(1)

Summary Introduction

To draw: Simple sketches showing a person stretching a spring.

Introduction: Energy is necessary for all living organisms to perform their life processes. Living cells obtain energy in a different form and they also have the mechanisms to alter energy from one form to another form. Energy is defined as the capability to do work.

(2)

Summary Introduction

To draw: Simple sketches showing the stretched spring.

Introduction: Energy is necessary for all living organisms to perform their life processes. Living cells obtain energy in a different form and they also have the mechanisms to alter energy from one form to another form. Energy is defined as the capability to do work. When the work is done, energy is transferred from one form to another form or between systems. Energy is generally expressed in units of work that is kilojoules (kJ). Energy can also be expressed in units of heat energy, that is, kilocalories (kcal).

(3)

Summary Introduction

To draw: Simple sketches showing the release of tension.

Introduction: Energy is necessary for all living organisms to perform their life processes. Living cells obtain energy in a different form and they also have the mechanisms to alter energy from one form to another form. Energy is defined as the capability to do work. When the work is done, energy is transferred from one form to another form or between systems. Energy is generally expressed in units of work that is kilojoules (kJ). Energy can also be expressed in units of heat energy, that is, kilocalories (kcal).

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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?   2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?   3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol   -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes.   Questions: 1) What differences would you expect to see between the…
Overview of Transformation Protocol   -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes.   Questions: 1)What is the selectable marker in this experiment? How…
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