The signal transduction pathway of Escherichia coli in response to a hypertonic solution, with the help of a diagram. Given: The labeled P-EnvZ was incubated with OmpR protein and the samples were tested for the presence of fluorescence at different time intervals. The results were recorded in Table 1 below: Table 1: Labeled proteins observed at different intervals after the incubation of 32 P-EnvZ with OmpR. Duration after mixing 32 P-EnvZ and OmpR (min) 0 2 5 10 Presence of 32 P label in EnvZ High Medium Low Absent Presence of 32 P label in OmpR Absent Low Medium High Introduction: Cells activate different genes under a specific situation. The bacterial cells like E. coli synthesize high OmpC protein levels in hypertonic solutions, as compared to hypotonic or isotonic solutions. Proteins, like EnvZ which is a membrane protein, get phosphorylated and also function as a protein kinase. EnvZ also phosphorylates proteins, like OmpR, which is a cytoplasm protein.

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Answer 1

Question

Chapter 7, Problem 4Q

Summary Introduction

To review:

The signal transduction pathway of Escherichia coli in response to a hypertonic solution, with the help of a diagram.

Given:

The labeled P-EnvZ was incubated with OmpR protein and the samples were tested for the presence of fluorescence at different time intervals. The results were recorded in Table 1 below:

Table 1: Labeled proteins observed at different intervals after the incubation of ³²P-EnvZ with OmpR.

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Introduction:

Cells activate different genes under a specific situation. The bacterial cells like E. coli synthesize high OmpC protein levels in hypertonic solutions, as compared to hypotonic or isotonic solutions. Proteins, like EnvZ which is a membrane protein, get phosphorylated and also function as a protein kinase. EnvZ also phosphorylates proteins, like OmpR, which is a cytoplasm protein.

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Answer 2

Question

Chapter 7, Problem 4Q

Summary Introduction

To review:

The signal transduction pathway of Escherichia coli in response to a hypertonic solution, with the help of a diagram.

Given:

The labeled P-EnvZ was incubated with OmpR protein and the samples were tested for the presence of fluorescence at different time intervals. The results were recorded in Table 1 below:

Table 1: Labeled proteins observed at different intervals after the incubation of ³²P-EnvZ with OmpR.

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Introduction:

Cells activate different genes under a specific situation. The bacterial cells like E. coli synthesize high OmpC protein levels in hypertonic solutions, as compared to hypotonic or isotonic solutions. Proteins, like EnvZ which is a membrane protein, get phosphorylated and also function as a protein kinase. EnvZ also phosphorylates proteins, like OmpR, which is a cytoplasm protein.

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Answer 3

Question

Chapter 7, Problem 4Q

Summary Introduction

To review:

The signal transduction pathway of Escherichia coli in response to a hypertonic solution, with the help of a diagram.

Given:

The labeled P-EnvZ was incubated with OmpR protein and the samples were tested for the presence of fluorescence at different time intervals. The results were recorded in Table 1 below:

Table 1: Labeled proteins observed at different intervals after the incubation of ³²P-EnvZ with OmpR.

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Introduction:

Cells activate different genes under a specific situation. The bacterial cells like E. coli synthesize high OmpC protein levels in hypertonic solutions, as compared to hypotonic or isotonic solutions. Proteins, like EnvZ which is a membrane protein, get phosphorylated and also function as a protein kinase. EnvZ also phosphorylates proteins, like OmpR, which is a cytoplasm protein.

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Answer 4

Question

Chapter 7, Problem 4Q

Summary Introduction

To review:

The signal transduction pathway of Escherichia coli in response to a hypertonic solution, with the help of a diagram.

Given:

The labeled P-EnvZ was incubated with OmpR protein and the samples were tested for the presence of fluorescence at different time intervals. The results were recorded in Table 1 below:

Table 1: Labeled proteins observed at different intervals after the incubation of ³²P-EnvZ with OmpR.

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Introduction:

Cells activate different genes under a specific situation. The bacterial cells like E. coli synthesize high OmpC protein levels in hypertonic solutions, as compared to hypotonic or isotonic solutions. Proteins, like EnvZ which is a membrane protein, get phosphorylated and also function as a protein kinase. EnvZ also phosphorylates proteins, like OmpR, which is a cytoplasm protein.

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Answer 5

Chapter 7, Problem 4Q

Summary Introduction

To review:

The signal transduction pathway of Escherichia coli in response to a hypertonic solution, with the help of a diagram.

Given:

The labeled P-EnvZ was incubated with OmpR protein and the samples were tested for the presence of fluorescence at different time intervals. The results were recorded in Table 1 below:

Table 1: Labeled proteins observed at different intervals after the incubation of ³²P-EnvZ with OmpR.

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Introduction:

Cells activate different genes under a specific situation. The bacterial cells like E. coli synthesize high OmpC protein levels in hypertonic solutions, as compared to hypotonic or isotonic solutions. Proteins, like EnvZ which is a membrane protein, get phosphorylated and also function as a protein kinase. EnvZ also phosphorylates proteins, like OmpR, which is a cytoplasm protein.

Answer 6

Chapter 7, Problem 4Q

Summary Introduction

To review:

The signal transduction pathway of Escherichia coli in response to a hypertonic solution, with the help of a diagram.

Given:

The labeled P-EnvZ was incubated with OmpR protein and the samples were tested for the presence of fluorescence at different time intervals. The results were recorded in Table 1 below:

Table 1: Labeled proteins observed at different intervals after the incubation of ³²P-EnvZ with OmpR.

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Introduction:

Cells activate different genes under a specific situation. The bacterial cells like E. coli synthesize high OmpC protein levels in hypertonic solutions, as compared to hypotonic or isotonic solutions. Proteins, like EnvZ which is a membrane protein, get phosphorylated and also function as a protein kinase. EnvZ also phosphorylates proteins, like OmpR, which is a cytoplasm protein.

Answer 7

Chapter 7, Problem 4Q

Summary Introduction

To review:

The signal transduction pathway of Escherichia coli in response to a hypertonic solution, with the help of a diagram.

Given:

The labeled P-EnvZ was incubated with OmpR protein and the samples were tested for the presence of fluorescence at different time intervals. The results were recorded in Table 1 below:

Table 1: Labeled proteins observed at different intervals after the incubation of ³²P-EnvZ with OmpR.

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Introduction:

Cells activate different genes under a specific situation. The bacterial cells like E. coli synthesize high OmpC protein levels in hypertonic solutions, as compared to hypotonic or isotonic solutions. Proteins, like EnvZ which is a membrane protein, get phosphorylated and also function as a protein kinase. EnvZ also phosphorylates proteins, like OmpR, which is a cytoplasm protein.

Answer 8

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Answer 10

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Answer 11

Ch. 7.1 - Prob. 1R Ch. 7.1 - Prob. 2R Ch. 7.1 - Prob. 3R Ch. 7.2 - Prob. 1R Ch. 7.2 - Prob. 2R Ch. 7.2 - Prob. 3R Ch. 7.3 - Prob. 1R Ch. 7.3 - Prob. 2R Ch. 7.3 - Prob. 3R Ch. 7.4 - Prob. 1R

Solution Summary: The author describes the signal transduction pathway of Escherichia coli in response to a hypertonic solution.

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Chapter 7 Solutions

	Duration after mixing ³²P-EnvZ and OmpR (min)
	0	2	5	10
Presence of ³²P label in EnvZ	High	Medium	Low	Absent
Presence of ³²P label in OmpR	Absent	Low	Medium	High