The roles of OmpR and EnvZ. Introduction: A single-celled organism like Escherichia coli has to survive in harsh conditions. For this, they activate different genes according to the situation. For example, expression of the high amount of OmpC in hypertonic solutions as compared to isotonic or hypotonic solutions. Other proteins like membrane protein (EnvZ) get phosphorylated by protein kinase in the signal transduction. The incubation of cytoplasm protein (OmpR) and membrane protein (EnvZ) with 32 P (phosphorus)-radiolabeled ATP is already given as Table A Incubation of EnvZ with 32 P-ATP Incubation of OmpR with 32 P-ATP Incorporation of 32 P label into protein High Absent The incubation of P-EnvZ with OmpR and testing the samples at different time intervals give following results: Table B Duration after mixing 32 P-EnvZ and OmpR (min) 0 2 5 10 Presence of 32 P label in EnvZ High Medium Low Absent Presence of 32 P label in OmpR Absent Low Medium High

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Answer 1

Question

Chapter 7, Problem 2Q

Summary Introduction

To review:

The roles of OmpR and EnvZ.

Introduction:

A single-celled organism like Escherichia coli has to survive in harsh conditions. For this, they activate different genes according to the situation. For example, expression of the high amount of OmpC in hypertonic solutions as compared to isotonic or hypotonic solutions. Other proteins like membrane protein (EnvZ) get phosphorylated by protein kinase in the signal transduction.

The incubation of cytoplasm protein (OmpR) and membrane protein (EnvZ) with ³²P (phosphorus)-radiolabeled ATP is already given as

Table A

Incubation of EnvZ with ³²P-ATP Incubation of OmpR with ³²P-ATP

Incorporation of ³²P label into protein High Absent

The incubation of P-EnvZ with OmpR and testing the samples at different time intervals give following results:

Table B

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

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Answer 2

Question

Chapter 7, Problem 2Q

Summary Introduction

To review:

The roles of OmpR and EnvZ.

Introduction:

A single-celled organism like Escherichia coli has to survive in harsh conditions. For this, they activate different genes according to the situation. For example, expression of the high amount of OmpC in hypertonic solutions as compared to isotonic or hypotonic solutions. Other proteins like membrane protein (EnvZ) get phosphorylated by protein kinase in the signal transduction.

The incubation of cytoplasm protein (OmpR) and membrane protein (EnvZ) with ³²P (phosphorus)-radiolabeled ATP is already given as

Table A

Incubation of EnvZ with ³²P-ATP Incubation of OmpR with ³²P-ATP

Incorporation of ³²P label into protein High Absent

The incubation of P-EnvZ with OmpR and testing the samples at different time intervals give following results:

Table B

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

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Answer 3

Question

Chapter 7, Problem 2Q

Summary Introduction

To review:

The roles of OmpR and EnvZ.

Introduction:

A single-celled organism like Escherichia coli has to survive in harsh conditions. For this, they activate different genes according to the situation. For example, expression of the high amount of OmpC in hypertonic solutions as compared to isotonic or hypotonic solutions. Other proteins like membrane protein (EnvZ) get phosphorylated by protein kinase in the signal transduction.

The incubation of cytoplasm protein (OmpR) and membrane protein (EnvZ) with ³²P (phosphorus)-radiolabeled ATP is already given as

Table A

Incubation of EnvZ with ³²P-ATP Incubation of OmpR with ³²P-ATP

Incorporation of ³²P label into protein High Absent

The incubation of P-EnvZ with OmpR and testing the samples at different time intervals give following results:

Table B

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Expert Solution & Answer

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Answer 4

Question

Chapter 7, Problem 2Q

Summary Introduction

To review:

The roles of OmpR and EnvZ.

Introduction:

A single-celled organism like Escherichia coli has to survive in harsh conditions. For this, they activate different genes according to the situation. For example, expression of the high amount of OmpC in hypertonic solutions as compared to isotonic or hypotonic solutions. Other proteins like membrane protein (EnvZ) get phosphorylated by protein kinase in the signal transduction.

The incubation of cytoplasm protein (OmpR) and membrane protein (EnvZ) with ³²P (phosphorus)-radiolabeled ATP is already given as

Table A

Incubation of EnvZ with ³²P-ATP Incubation of OmpR with ³²P-ATP

Incorporation of ³²P label into protein High Absent

The incubation of P-EnvZ with OmpR and testing the samples at different time intervals give following results:

Table B

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Expert Solution & Answer

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Answer 5

Chapter 7, Problem 2Q

Summary Introduction

To review:

The roles of OmpR and EnvZ.

Introduction:

A single-celled organism like Escherichia coli has to survive in harsh conditions. For this, they activate different genes according to the situation. For example, expression of the high amount of OmpC in hypertonic solutions as compared to isotonic or hypotonic solutions. Other proteins like membrane protein (EnvZ) get phosphorylated by protein kinase in the signal transduction.

The incubation of cytoplasm protein (OmpR) and membrane protein (EnvZ) with ³²P (phosphorus)-radiolabeled ATP is already given as

Table A

Incubation of EnvZ with ³²P-ATP Incubation of OmpR with ³²P-ATP

Incorporation of ³²P label into protein High Absent

The incubation of P-EnvZ with OmpR and testing the samples at different time intervals give following results:

Table B

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Answer 6

Chapter 7, Problem 2Q

Summary Introduction

To review:

The roles of OmpR and EnvZ.

Introduction:

A single-celled organism like Escherichia coli has to survive in harsh conditions. For this, they activate different genes according to the situation. For example, expression of the high amount of OmpC in hypertonic solutions as compared to isotonic or hypotonic solutions. Other proteins like membrane protein (EnvZ) get phosphorylated by protein kinase in the signal transduction.

The incubation of cytoplasm protein (OmpR) and membrane protein (EnvZ) with ³²P (phosphorus)-radiolabeled ATP is already given as

Table A

Incubation of EnvZ with ³²P-ATP Incubation of OmpR with ³²P-ATP

Incorporation of ³²P label into protein High Absent

The incubation of P-EnvZ with OmpR and testing the samples at different time intervals give following results:

Table B

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Answer 7

Chapter 7, Problem 2Q

Summary Introduction

To review:

The roles of OmpR and EnvZ.

Introduction:

A single-celled organism like Escherichia coli has to survive in harsh conditions. For this, they activate different genes according to the situation. For example, expression of the high amount of OmpC in hypertonic solutions as compared to isotonic or hypotonic solutions. Other proteins like membrane protein (EnvZ) get phosphorylated by protein kinase in the signal transduction.

The incubation of cytoplasm protein (OmpR) and membrane protein (EnvZ) with ³²P (phosphorus)-radiolabeled ATP is already given as

Table A

Incubation of EnvZ with ³²P-ATP Incubation of OmpR with ³²P-ATP

Incorporation of ³²P label into protein High Absent

The incubation of P-EnvZ with OmpR and testing the samples at different time intervals give following results:

Table B

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Answer 8

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Answer 9

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Answer 10

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Answer 11

Ch. 7.1 - Prob. 1R Ch. 7.1 - Prob. 2R Ch. 7.1 - Prob. 3R Ch. 7.2 - Prob. 1R Ch. 7.2 - Prob. 2R Ch. 7.2 - Prob. 3R Ch. 7.3 - Prob. 1R Ch. 7.3 - Prob. 2R Ch. 7.3 - Prob. 3R Ch. 7.4 - Prob. 1R

Solution Summary: The author explains the roles of OmpR and EnvZ in Escherichia coli.

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Chapter 7 Solutions

	Incubation of EnvZ with ³²P-ATP	Incubation of OmpR with ³²P-ATP
Incorporation of ³²P label into protein	High	Absent

Duration after mixing ³²P-EnvZ and OmpR (min)
	0	2	5	10
Presence of ³²P label in EnvZ	High	Medium	Low	Absent
Presence of ³²P label in OmpR	Absent	Low	Medium	High