The roles of OmpR and EnvZ. Introduction: A single-celled organism like Escherichia coli has to survive in harsh conditions. For this, they activate different genes according to the situation. For example, expression of the high amount of OmpC in hypertonic solutions as compared to isotonic or hypotonic solutions. Other proteins like membrane protein (EnvZ) get phosphorylated by protein kinase in the signal transduction. The incubation of cytoplasm protein (OmpR) and membrane protein (EnvZ) with 32 P (phosphorus)-radiolabeled ATP is already given as Table A Incubation of EnvZ with 32 P-ATP Incubation of OmpR with 32 P-ATP Incorporation of 32 P label into protein High Absent The incubation of P-EnvZ with OmpR and testing the samples at different time intervals give following results: Table B Duration after mixing 32 P-EnvZ and OmpR (min) 0 2 5 10 Presence of 32 P label in EnvZ High Medium Low Absent Presence of 32 P label in OmpR Absent Low Medium High

Question

Want to see the full answer?

Answer 1

Question

Chapter 7, Problem 2Q

Summary Introduction

To review:

The roles of OmpR and EnvZ.

Introduction:

A single-celled organism like Escherichia coli has to survive in harsh conditions. For this, they activate different genes according to the situation. For example, expression of the high amount of OmpC in hypertonic solutions as compared to isotonic or hypotonic solutions. Other proteins like membrane protein (EnvZ) get phosphorylated by protein kinase in the signal transduction.

The incubation of cytoplasm protein (OmpR) and membrane protein (EnvZ) with ³²P (phosphorus)-radiolabeled ATP is already given as

Table A

Incubation of EnvZ with ³²P-ATP Incubation of OmpR with ³²P-ATP

Incorporation of ³²P label into protein High Absent

The incubation of P-EnvZ with OmpR and testing the samples at different time intervals give following results:

Table B

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

Kinetics: One-Compartment First-Order Absorption 1. In vivo testing provides valuable insight into a drug’s kinetics. Assessing drug kinetics following multiple routes of administration provides greater insight than a single route of administration alone. The following data was collected in 250-g rats following bolus IV, oral (PO), and intraperitoneal (ip) administration. Using this data and set of graphs, determine:(calculate for each variable) (a) k, C0, V, and AUC* for the bolus iv data (b) k, ka, B1, and AUC* for the po data c) k, ka, B1, and AUC* for the ip data (d) relative bioavailability for po vs ip, Fpo/Fip (e)absolute ip bioavailability, Fip (f) absolute po bioavailability, Fpo

3. A promising new drug is being evaluated in human trials. Based on preliminary human tests, this drug is most effective when plasma levels exceed 30 mg/L. Measurements from preliminary tests indicate the following human pharmacokinetic parameter values: t1/2,elim = 4.6hr, t1/2,abs = 0.34hr, VD = 0.29 L/kg, Foral = 72%. Based on these parameters, estimate the following if a 49 kg woman were to receive a 1000mg oral dose of this drug: (a) Estimate the plasma concentration of the drug at 1hr, 6 hr, and 20hr after taking the drug ( Concentration estimate) (b) Estimate the time for maximum plasma concentration (tmax). (c) Estimate the maximum plasma concentration (Cmax). (d) Estimate the time at which the plasma level first rises above 30 mg/L. (Note this is a trial and error problem where you must guess a time, plug it into the concentration equation, and determine if it is close to 30 mg/L. Hint: based on part (a) it should be apparent that the answer is less than 1hr.) (e)…

List substitutions in your diet you could make to improve it based on what you know now about a balanced diet. For instance, if you like to drink soda, you might substitute skim milk or water for some of the soft drinks you consumed. List the item you wish to replace with the new item and what you hope to accomplish with that substitution. Be sure to choose foods you know that you'd enjoy and you consider more "healthful." If you feel your diet is already balanced, describe how you accomplish your balanced intake and when you began eating this way.

Answer 2

Question

Chapter 7, Problem 2Q

Summary Introduction

To review:

The roles of OmpR and EnvZ.

Introduction:

A single-celled organism like Escherichia coli has to survive in harsh conditions. For this, they activate different genes according to the situation. For example, expression of the high amount of OmpC in hypertonic solutions as compared to isotonic or hypotonic solutions. Other proteins like membrane protein (EnvZ) get phosphorylated by protein kinase in the signal transduction.

The incubation of cytoplasm protein (OmpR) and membrane protein (EnvZ) with ³²P (phosphorus)-radiolabeled ATP is already given as

Table A

Incubation of EnvZ with ³²P-ATP Incubation of OmpR with ³²P-ATP

Incorporation of ³²P label into protein High Absent

The incubation of P-EnvZ with OmpR and testing the samples at different time intervals give following results:

Table B

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 3

Question

Chapter 7, Problem 2Q

Summary Introduction

To review:

The roles of OmpR and EnvZ.

Introduction:

A single-celled organism like Escherichia coli has to survive in harsh conditions. For this, they activate different genes according to the situation. For example, expression of the high amount of OmpC in hypertonic solutions as compared to isotonic or hypotonic solutions. Other proteins like membrane protein (EnvZ) get phosphorylated by protein kinase in the signal transduction.

The incubation of cytoplasm protein (OmpR) and membrane protein (EnvZ) with ³²P (phosphorus)-radiolabeled ATP is already given as

Table A

Incubation of EnvZ with ³²P-ATP Incubation of OmpR with ³²P-ATP

Incorporation of ³²P label into protein High Absent

The incubation of P-EnvZ with OmpR and testing the samples at different time intervals give following results:

Table B

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 7, Problem 2Q

Summary Introduction

To review:

The roles of OmpR and EnvZ.

Introduction:

A single-celled organism like Escherichia coli has to survive in harsh conditions. For this, they activate different genes according to the situation. For example, expression of the high amount of OmpC in hypertonic solutions as compared to isotonic or hypotonic solutions. Other proteins like membrane protein (EnvZ) get phosphorylated by protein kinase in the signal transduction.

The incubation of cytoplasm protein (OmpR) and membrane protein (EnvZ) with ³²P (phosphorus)-radiolabeled ATP is already given as

Table A

Incubation of EnvZ with ³²P-ATP Incubation of OmpR with ³²P-ATP

Incorporation of ³²P label into protein High Absent

The incubation of P-EnvZ with OmpR and testing the samples at different time intervals give following results:

Table B

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 5

Chapter 7, Problem 2Q

Summary Introduction

To review:

The roles of OmpR and EnvZ.

Introduction:

A single-celled organism like Escherichia coli has to survive in harsh conditions. For this, they activate different genes according to the situation. For example, expression of the high amount of OmpC in hypertonic solutions as compared to isotonic or hypotonic solutions. Other proteins like membrane protein (EnvZ) get phosphorylated by protein kinase in the signal transduction.

The incubation of cytoplasm protein (OmpR) and membrane protein (EnvZ) with ³²P (phosphorus)-radiolabeled ATP is already given as

Table A

Incubation of EnvZ with ³²P-ATP Incubation of OmpR with ³²P-ATP

Incorporation of ³²P label into protein High Absent

The incubation of P-EnvZ with OmpR and testing the samples at different time intervals give following results:

Table B

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Answer 6

Chapter 7, Problem 2Q

Summary Introduction

To review:

The roles of OmpR and EnvZ.

Introduction:

A single-celled organism like Escherichia coli has to survive in harsh conditions. For this, they activate different genes according to the situation. For example, expression of the high amount of OmpC in hypertonic solutions as compared to isotonic or hypotonic solutions. Other proteins like membrane protein (EnvZ) get phosphorylated by protein kinase in the signal transduction.

The incubation of cytoplasm protein (OmpR) and membrane protein (EnvZ) with ³²P (phosphorus)-radiolabeled ATP is already given as

Table A

Incubation of EnvZ with ³²P-ATP Incubation of OmpR with ³²P-ATP

Incorporation of ³²P label into protein High Absent

The incubation of P-EnvZ with OmpR and testing the samples at different time intervals give following results:

Table B

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Answer 7

Chapter 7, Problem 2Q

Summary Introduction

To review:

The roles of OmpR and EnvZ.

Introduction:

A single-celled organism like Escherichia coli has to survive in harsh conditions. For this, they activate different genes according to the situation. For example, expression of the high amount of OmpC in hypertonic solutions as compared to isotonic or hypotonic solutions. Other proteins like membrane protein (EnvZ) get phosphorylated by protein kinase in the signal transduction.

The incubation of cytoplasm protein (OmpR) and membrane protein (EnvZ) with ³²P (phosphorus)-radiolabeled ATP is already given as

Table A

Incubation of EnvZ with ³²P-ATP Incubation of OmpR with ³²P-ATP

Incorporation of ³²P label into protein High Absent

The incubation of P-EnvZ with OmpR and testing the samples at different time intervals give following results:

Table B

Duration after mixing ³²P-EnvZ and OmpR (min)

0 2 5 10

Presence of ³²P label in EnvZ High Medium Low Absent

Presence of ³²P label in OmpR Absent Low Medium High

Answer 8

Expert Solution & Answer

Answer 9

Expert Solution & Answer

Answer 10

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 11

Ch. 7.1 - Prob. 1R Ch. 7.1 - Prob. 2R Ch. 7.1 - Prob. 3R Ch. 7.2 - Prob. 1R Ch. 7.2 - Prob. 2R Ch. 7.2 - Prob. 3R Ch. 7.3 - Prob. 1R Ch. 7.3 - Prob. 2R Ch. 7.3 - Prob. 3R Ch. 7.4 - Prob. 1R

Solution Summary: The author explains the roles of OmpR and EnvZ in Escherichia coli.

Concept explainers

Videos

Want to see the full answer?

Chapter 7 Solutions

	Incubation of EnvZ with ³²P-ATP	Incubation of OmpR with ³²P-ATP
Incorporation of ³²P label into protein	High	Absent

Duration after mixing ³²P-EnvZ and OmpR (min)
	0	2	5	10
Presence of ³²P label in EnvZ	High	Medium	Low	Absent
Presence of ³²P label in OmpR	Absent	Low	Medium	High