The evidence that suggest that protein composition in the plasma membrane of bacterial cell changes in response to changes in solute concentration of the environment. Introduction: Bacterial cells like Escherichia coli live in harsh conditions of the intestine in mammals including humans. When present in a hypertonic solution, the cells tend to desiccate; when present in hypotonic solution, the cells tend to swell. It was observed that, they still tend to survive in multiple environmental conditions and remain unaffected. Thus, in this way, they were able to carry out their role in the intestine.

Question

Want to see more full solutions like this?

Answer 1

Question

Chapter 7, Problem 1Q

Summary Introduction

To review:

The evidence that suggest that protein composition in the plasma membrane of bacterial cell changes in response to changes in solute concentration of the environment.

Introduction:

Bacterial cells like Escherichia coli live in harsh conditions of the intestine in mammals including humans. When present in a hypertonic solution, the cells tend to desiccate; when present in hypotonic solution, the cells tend to swell. It was observed that, they still tend to survive in multiple environmental conditions and remain unaffected. Thus, in this way, they were able to carry out their role in the intestine.

Expert Solution & Answer

Explanation of Solution

For the determination of changes in bacterial cell that is adapted to different conditions, the scientists placed the bacterial cells in two different solutions: hypertonic and isotonic. In the first solution (hypertonic), the solute concentration was higher than the solute concentration present inside the cell. The second solution was of the same tonicity as that of the bacterial cell.

In the hypertonic solution, the bacterial cells expressed a protein named OmpC in their membranes. This protein was separated by polyacrylamide gel electrophoresis (PAGE) technique. The protein was analyzed with the help of a western blot. In western blot, the darker stain of OmpC extracted from hypertonic solutions represented its high quantity in those cells.

Conclusion

Thus, the gel electrophoresis was used to detect the presence of a protein in the cells. Thus, OmpC is present in higher quantities in the cell membranes in the hypertonic solution as compared to the isotonic or hypotonic solutions. This experiment provided evidence for the changes in the membrane proteins with altering environment.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Students have asked these similar questions

What is the concept "calories consumed must equal calories burned" in regrads to nutrition?

You intend to insert patched dominant negative DNA into the left half of the neural tube of a chick. 1) Which side of the neural tube would you put the positive electrode to ensure that the DNA ends up on the left side? 2) What would be the internal (within the embryo) control for this experiment? 3) How can you be sure that the electroporation method itself is not impacting the embryo? 4) What would you do to ensure that the electroporation is working? How can you tell?

Describe a method to document the diffusion path and gradient of Sonic Hedgehog through the chicken embryo. If modifying the protein, what is one thing you have to consider in regards to maintaining the protein’s function?

Answer 2

Question

Chapter 7, Problem 1Q

Summary Introduction

To review:

The evidence that suggest that protein composition in the plasma membrane of bacterial cell changes in response to changes in solute concentration of the environment.

Introduction:

Bacterial cells like Escherichia coli live in harsh conditions of the intestine in mammals including humans. When present in a hypertonic solution, the cells tend to desiccate; when present in hypotonic solution, the cells tend to swell. It was observed that, they still tend to survive in multiple environmental conditions and remain unaffected. Thus, in this way, they were able to carry out their role in the intestine.

Expert Solution & Answer

Explanation of Solution

For the determination of changes in bacterial cell that is adapted to different conditions, the scientists placed the bacterial cells in two different solutions: hypertonic and isotonic. In the first solution (hypertonic), the solute concentration was higher than the solute concentration present inside the cell. The second solution was of the same tonicity as that of the bacterial cell.

In the hypertonic solution, the bacterial cells expressed a protein named OmpC in their membranes. This protein was separated by polyacrylamide gel electrophoresis (PAGE) technique. The protein was analyzed with the help of a western blot. In western blot, the darker stain of OmpC extracted from hypertonic solutions represented its high quantity in those cells.

Conclusion

Thus, the gel electrophoresis was used to detect the presence of a protein in the cells. Thus, OmpC is present in higher quantities in the cell membranes in the hypertonic solution as compared to the isotonic or hypotonic solutions. This experiment provided evidence for the changes in the membrane proteins with altering environment.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 3

Question

Chapter 7, Problem 1Q

Summary Introduction

To review:

The evidence that suggest that protein composition in the plasma membrane of bacterial cell changes in response to changes in solute concentration of the environment.

Introduction:

Bacterial cells like Escherichia coli live in harsh conditions of the intestine in mammals including humans. When present in a hypertonic solution, the cells tend to desiccate; when present in hypotonic solution, the cells tend to swell. It was observed that, they still tend to survive in multiple environmental conditions and remain unaffected. Thus, in this way, they were able to carry out their role in the intestine.

Expert Solution & Answer

Explanation of Solution

For the determination of changes in bacterial cell that is adapted to different conditions, the scientists placed the bacterial cells in two different solutions: hypertonic and isotonic. In the first solution (hypertonic), the solute concentration was higher than the solute concentration present inside the cell. The second solution was of the same tonicity as that of the bacterial cell.

In the hypertonic solution, the bacterial cells expressed a protein named OmpC in their membranes. This protein was separated by polyacrylamide gel electrophoresis (PAGE) technique. The protein was analyzed with the help of a western blot. In western blot, the darker stain of OmpC extracted from hypertonic solutions represented its high quantity in those cells.

Conclusion

Thus, the gel electrophoresis was used to detect the presence of a protein in the cells. Thus, OmpC is present in higher quantities in the cell membranes in the hypertonic solution as compared to the isotonic or hypotonic solutions. This experiment provided evidence for the changes in the membrane proteins with altering environment.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Question

Chapter 7, Problem 1Q

Summary Introduction

To review:

The evidence that suggest that protein composition in the plasma membrane of bacterial cell changes in response to changes in solute concentration of the environment.

Introduction:

Bacterial cells like Escherichia coli live in harsh conditions of the intestine in mammals including humans. When present in a hypertonic solution, the cells tend to desiccate; when present in hypotonic solution, the cells tend to swell. It was observed that, they still tend to survive in multiple environmental conditions and remain unaffected. Thus, in this way, they were able to carry out their role in the intestine.

Expert Solution & Answer

Explanation of Solution

For the determination of changes in bacterial cell that is adapted to different conditions, the scientists placed the bacterial cells in two different solutions: hypertonic and isotonic. In the first solution (hypertonic), the solute concentration was higher than the solute concentration present inside the cell. The second solution was of the same tonicity as that of the bacterial cell.

In the hypertonic solution, the bacterial cells expressed a protein named OmpC in their membranes. This protein was separated by polyacrylamide gel electrophoresis (PAGE) technique. The protein was analyzed with the help of a western blot. In western blot, the darker stain of OmpC extracted from hypertonic solutions represented its high quantity in those cells.

Conclusion

Thus, the gel electrophoresis was used to detect the presence of a protein in the cells. Thus, OmpC is present in higher quantities in the cell membranes in the hypertonic solution as compared to the isotonic or hypotonic solutions. This experiment provided evidence for the changes in the membrane proteins with altering environment.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Chapter 7, Problem 1Q

Summary Introduction

To review:

The evidence that suggest that protein composition in the plasma membrane of bacterial cell changes in response to changes in solute concentration of the environment.

Introduction:

Bacterial cells like Escherichia coli live in harsh conditions of the intestine in mammals including humans. When present in a hypertonic solution, the cells tend to desiccate; when present in hypotonic solution, the cells tend to swell. It was observed that, they still tend to survive in multiple environmental conditions and remain unaffected. Thus, in this way, they were able to carry out their role in the intestine.

Expert Solution & Answer

Explanation of Solution

For the determination of changes in bacterial cell that is adapted to different conditions, the scientists placed the bacterial cells in two different solutions: hypertonic and isotonic. In the first solution (hypertonic), the solute concentration was higher than the solute concentration present inside the cell. The second solution was of the same tonicity as that of the bacterial cell.

In the hypertonic solution, the bacterial cells expressed a protein named OmpC in their membranes. This protein was separated by polyacrylamide gel electrophoresis (PAGE) technique. The protein was analyzed with the help of a western blot. In western blot, the darker stain of OmpC extracted from hypertonic solutions represented its high quantity in those cells.

Conclusion

Thus, the gel electrophoresis was used to detect the presence of a protein in the cells. Thus, OmpC is present in higher quantities in the cell membranes in the hypertonic solution as compared to the isotonic or hypotonic solutions. This experiment provided evidence for the changes in the membrane proteins with altering environment.

Answer 6

Chapter 7, Problem 1Q

Summary Introduction

To review:

The evidence that suggest that protein composition in the plasma membrane of bacterial cell changes in response to changes in solute concentration of the environment.

Introduction:

Bacterial cells like Escherichia coli live in harsh conditions of the intestine in mammals including humans. When present in a hypertonic solution, the cells tend to desiccate; when present in hypotonic solution, the cells tend to swell. It was observed that, they still tend to survive in multiple environmental conditions and remain unaffected. Thus, in this way, they were able to carry out their role in the intestine.

Expert Solution & Answer

Explanation of Solution

For the determination of changes in bacterial cell that is adapted to different conditions, the scientists placed the bacterial cells in two different solutions: hypertonic and isotonic. In the first solution (hypertonic), the solute concentration was higher than the solute concentration present inside the cell. The second solution was of the same tonicity as that of the bacterial cell.

In the hypertonic solution, the bacterial cells expressed a protein named OmpC in their membranes. This protein was separated by polyacrylamide gel electrophoresis (PAGE) technique. The protein was analyzed with the help of a western blot. In western blot, the darker stain of OmpC extracted from hypertonic solutions represented its high quantity in those cells.

Conclusion

Thus, the gel electrophoresis was used to detect the presence of a protein in the cells. Thus, OmpC is present in higher quantities in the cell membranes in the hypertonic solution as compared to the isotonic or hypotonic solutions. This experiment provided evidence for the changes in the membrane proteins with altering environment.

Answer 7

Chapter 7, Problem 1Q

Summary Introduction

To review:

The evidence that suggest that protein composition in the plasma membrane of bacterial cell changes in response to changes in solute concentration of the environment.

Introduction:

Bacterial cells like Escherichia coli live in harsh conditions of the intestine in mammals including humans. When present in a hypertonic solution, the cells tend to desiccate; when present in hypotonic solution, the cells tend to swell. It was observed that, they still tend to survive in multiple environmental conditions and remain unaffected. Thus, in this way, they were able to carry out their role in the intestine.

Answer 8

Expert Solution & Answer

Explanation of Solution

For the determination of changes in bacterial cell that is adapted to different conditions, the scientists placed the bacterial cells in two different solutions: hypertonic and isotonic. In the first solution (hypertonic), the solute concentration was higher than the solute concentration present inside the cell. The second solution was of the same tonicity as that of the bacterial cell.

In the hypertonic solution, the bacterial cells expressed a protein named OmpC in their membranes. This protein was separated by polyacrylamide gel electrophoresis (PAGE) technique. The protein was analyzed with the help of a western blot. In western blot, the darker stain of OmpC extracted from hypertonic solutions represented its high quantity in those cells.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Conclusion

Thus, the gel electrophoresis was used to detect the presence of a protein in the cells. Thus, OmpC is present in higher quantities in the cell membranes in the hypertonic solution as compared to the isotonic or hypotonic solutions. This experiment provided evidence for the changes in the membrane proteins with altering environment.

Answer 12

Ch. 7.1 - Prob. 1R Ch. 7.1 - Prob. 2R Ch. 7.1 - Prob. 3R Ch. 7.2 - Prob. 1R Ch. 7.2 - Prob. 2R Ch. 7.2 - Prob. 3R Ch. 7.3 - Prob. 1R Ch. 7.3 - Prob. 2R Ch. 7.3 - Prob. 3R Ch. 7.4 - Prob. 1R

Videos

Explanation of Solution

Want to see more full solutions like this?

Chapter 7 Solutions