ANATOMY AND PHYSIOLOGY: AN INTEGRATIVE A
4th Edition
ISBN: 9781265955021
Author: McKinley
Publisher: MCG
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Chapter 7, Problem 19DYKB
Summary Introduction
To analyze:
The way parathyroid hormone regulates blood calcium concentration.
Introduction:
PTH is a chemical messenger called a hormone usually secreted by the parathyroid glands that seek to regulate the level of calcium in the blood. It oversees vitamin D and phosphorus levels too. When the body generates fluctuation in parathyroid hormone, it can cause symptoms that are associated with elevated calcium levels in the bloodstream.
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Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
Chapter 7 Solutions
ANATOMY AND PHYSIOLOGY: AN INTEGRATIVE A
Ch. 7.1 - Describe the composition of compact bone and...Ch. 7.1 - In what three locations of the body do you find...Ch. 7.2 - What two minerals are stored in bone, and what are...Ch. 7.2 - What are several examples of flat bones in the...Ch. 7.2 - Prob. 5WDYLCh. 7.2 - Prob. 6WDYLCh. 7.2 - Where is red bone marrow found in the adult...Ch. 7.2 - What are the functions of the osteoprogenitor...Ch. 7.2 - What organic and inorganic substances compose bone...Ch. 7.2 - What are the major components of an osteon?
Ch. 7.2 - What are the primary ways that hyaline cartilage...Ch. 7.3 - Where do interstitial and appositional growth of...Ch. 7.4 - Prob. 13WDYLCh. 7.4 - Briefly describe the process by which a long bone...Ch. 7.5 - Prob. 15WDYLCh. 7.5 - What is bone remodeling, where does it occur, and...Ch. 7.5 - Prob. 17WDYLCh. 7.6 - Prob. 18WDYLCh. 7.6 - Prob. 19WDYLCh. 7.6 - Prob. 20WDYLCh. 7.7 - Prob. 21WDYLCh. 7.8 - Prob. 22WDYLCh. 7 - _____ 1. Which bone is formed from intramembranous...Ch. 7 - Prob. 2DYKBCh. 7 - Prob. 3DYKBCh. 7 - Prob. 4DYKBCh. 7 - Prob. 5DYKBCh. 7 - _____ 6. Which long bone structure is correctly...Ch. 7 - _____ 7. Which statement is correct about an...Ch. 7 - Prob. 8DYKBCh. 7 - Prob. 9DYKBCh. 7 - Prob. 10DYKBCh. 7 - Prob. 11DYKBCh. 7 - Prob. 12DYKBCh. 7 - Prob. 13DYKBCh. 7 - Prob. 14DYKBCh. 7 - Prob. 15DYKBCh. 7 - Prob. 16DYKBCh. 7 - Prob. 17DYKBCh. 7 - Prob. 18DYKBCh. 7 - Prob. 19DYKBCh. 7 - Prob. 20DYKBCh. 7 - Prob. 1CALCh. 7 - Prob. 2CALCh. 7 - Prob. 3CALCh. 7 - To identify the approximate age of skeletal...Ch. 7 - In your anatomy and physiology laboratory, you...Ch. 7 - The traditional surgical procedure to treat...Ch. 7 - A fireman fell from a ladder while fighting a fire...Ch. 7 - Prob. 3CSL
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forwardIn oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the reaction, OTMPD is electron acceptor O cytochrome c is the electron source oxygen is terminal electron acceptor OH2 produced is electron donorarrow_forward
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