Chapter 6, Problem 14P

Summary Introduction

To analyze:

Seven deletion mutations are tested for their ability to form wild-type recombinants with five point mutations as shown in the table below:

	Deletion mutation
Point mutation	$\text{1}$	$\text{2}$	$\text{3}$	$\text{4}$	$\text{5}$	$\text{6}$	$\text{7}$
a	-	+	-	-	+	+	-
b	+	+	+	-	+	-	-
c	+	+	+	+	-	-	-
d	-	+	+	-	+	-	-
e	+	-	-	-	+	+	-

Using the data from the table, construct a genetic map of the order of point mutations and indicate the segment deleted by each deletion mutation.

Introduction:

The symbol ‘$\text{+}$’ indicates that a wild type recombination occurs and ‘$\text{-}$’ indicates that wild type recombinations are not formed. The result of insertion or deletion of a particular gene from the genome of an organism results in recombination. Point mutation is the one which only affects a single nucleotide in a particular gene of a genome. They include insertions or deletions of a single base pair. Point mutations are revertible i.e., wild type forms can be produced after reversion.