Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Question
Chapter 6, Problem 26P
Summary Introduction
To analyze:
To devise an experiment to identify Auxotrophic bacteria that are unable to produce lysine (
Introduction:
Replica plating, a simple technique to transfer few cells from each bacterial colony in a plate to another plate, will be useful to identify auxotrophic bacteria in a pool of prototrophic bacteria.
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Chapter 6 Solutions
Genetic Analysis: An Integrated Approach (3rd Edition)
Ch. 6 - For bacteria that are F+, Hfr, F', and F-, perform...Ch. 6 - The flow diagram identifies relationships between...Ch. 6 - Conjugation between an Hfr cell and an F-cell does...Ch. 6 - Bacteria transfer genes by conjugation,...Ch. 6 - Explain the importance of the following features...Ch. 6 - Prob. 6PCh. 6 - Describe what is meant by the term site-specific...Ch. 6 - What is a prophage, and how is a prophage formed?Ch. 6 - How is the frequency of cotransduction related to...Ch. 6 - Describe the differences between genetic...
Ch. 6 - Among the mechanisms of gene transfer in bacteria,...Ch. 6 - What is lateral gene transfer? How might it take...Ch. 6 - Lateral gene transfer is thought to have played a...Ch. 6 - Prob. 14PCh. 6 - A 2013 CDC report identified the practice of...Ch. 6 - Hfr strains that differ in integrated F factor...Ch. 6 - Five Hfr strains from the same bacterial species...Ch. 6 - An interrupted mating study is carried out on Hfr...Ch. 6 - An Hfr strain with the genotype cys+leu+met+strS...Ch. 6 - A triple-auxotrophic strain of E. coli having the...Ch. 6 - Penicillin was first used in the 1940 s to treat...Ch. 6 - An attribute of growth behavior of eight...Ch. 6 - Synthesis of the amino acid histidine is a...Ch. 6 - The phage P1 is used as a generalized transducing...Ch. 6 - Prob. 25PCh. 6 - Prob. 26PCh. 6 - Look closely at the consolidated Hfr map and the...Ch. 6 - Fifty bacterial colonies are on a complete-medium...
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Similar questions
- Define the terms prototroph and auxotroph.arrow_forwardWhat is the genus and species of your bacteria? And What led you to confirm this result and why? Please discuss. ☆My bacteria is Alcaligenes faecalisarrow_forwardWhy is the use of auxotrophy not a good method for controllingthe growth of a genetically modified organism?arrow_forward
- Prototrophy is often the phenotype selected to detecttransformants. Prototrophic cells are used for donorDNA extraction; then this DNA is cloned and the clonesare added to an auxotrophic recipient culture. Successfultransformants are identified by plating the recipientculture on minimal medium and looking for colonies.What experimental design would you use to make surethat a colony that you hope is a transformant is not, infact,a. a prototrophic cell that has entered the recipientculture as a contaminant?b. a revertant (mutation back to prototrophy by a secondmutation in the originally mutated gene) of theauxotrophic mutation?arrow_forwardIn their Nobel Prize-winning experiments, Beadle and Tatum exposed fungal spores to X-rays to induce mutations. If the mutants had mutations in the arginine metabolic pathway, they would grow in ------minimal medium (wrong answer), but NOT in -------complete medium (wrong answer).arrow_forwardOne of the early results shows that the post-centrifugation pellet of encapsulated cells also contains EA1 and/or Sap. Why is this not proof that Bacillus anthracis cells have both an S-layer and a capsule simultaneously? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/arrow_forward
- One of the early results shows that the post-centrifugation pellet of encapsulated cells also contains EA1 and/or Sap. Why is this not proof that Bacillus anthracis cells have both an S-layer and a capsule simultaneously? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/arrow_forwardwhy Auxotrophs cannotsynthesize crucial complex compounds from simplematerials.?arrow_forwardsuggest a strategy that you might employ to isolate all of the genes involved in nitrogen fixation from Azobacter vinelandi assuming that you do not have nif genes from other microorganisms to use as hybridization probes and discuss whether it is possible to genetically engineer plants to fix nitrogen.arrow_forward
- "the supplement that restored growth would be the molecule that the mutant strain could not synthesize" Explain this statement ?arrow_forwardWhat is the difference between complete medium and minimal medium? How are complete media and minimal media to which one or more nutrients have been added (selective media) used to isolate auxotrophic mutants of bacteria?arrow_forwardYou have four strains, and you test for growth on the following medias: Rich, Minimal, Minimal + Lysine, Minimal + Histidine, and Minimal +Lysine and Histidine. The table below shows your results. Strain Rich 1 2 3 4 Minimal Min + Lysine Strain 2 Growth No Growth Growth Growth No Growth No Growth Strain 4 Growth Growth Strain 1 Lysine auxotroph only Growth Histidine auxotroph only Strain 3 is not an auxotroph, but a pro Min + Histidine No Growth Is an auxotroph, but not for ly ✓ No Growth Growth Determine whether each strain is auxotroph or a prototroph, and if an auxotroph what sort of auxotroph? Min + Lysine and Histidine Growth No Growth No Growth No Growth No Growth Growth Growth Growtharrow_forward
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