BIOLOGY >PRINT UPGRADE<
11th Edition
ISBN: 9780357091586
Author: Solomon
Publisher: CENGAGE L
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Chapter 47, Problem 9TYU
Summary Introduction
Introduction: The
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1) Given an mRNA with the following sequence, please translate the codons to a chain of amino acids. Use the codon chart below.5’AUG/CCU/GCU/UAC/CGG/GAG/UAA3’
“met-_________-_________-_________-_________-_________”STOP”
2) Assuming the original polypeptide chain below, match each type of point mutation with the polypeptide chain that results.
Original polypeptide: Pro-Thr-Ser-Leu-Leu-His-Asn
A. Missense
B. Silent
C. Nonsense
D. Frameshift
______ Pro-Thr-Ser-STOP
______ Pro-Thr-Ser-Leu-Ile-His-Asn
______ Pro-Thr-Ser-Leu-Leu-His-Asn
_______ Pro-Thr-His-Cys-Tyr-Thr
a) The relationship between the Hawaiian bobtail squid and V. fischeri is symbiotic where both species benefit. What is the benefit to each?
b) Why might quorum sensing be beneficial to pathogenic bacteria?
c) How might scientists use quorum sensing to treat bacterial infections?
a) What is the function of the disulfide bonds that form within and between the alpha and beta chains?
b) How does proinsulin differ from mature insulin?
c) What organelles are involved in forming mature insulin?
Chapter 47 Solutions
BIOLOGY >PRINT UPGRADE<
Ch. 47.1 - Prob. 1LOCh. 47.1 - Prob. 1CCh. 47.1 - Prob. 2CCh. 47.1 - Prob. 3CCh. 47.2 - Prob. 2LOCh. 47.2 - Prob. 3LOCh. 47.2 - Describe the structural adaptations that increase...Ch. 47.2 - Prob. 5LOCh. 47.2 - Imagine that you have just taken a bite of a...Ch. 47.2 - How are villi in the small intestine functionally...
Ch. 47.2 - What are the functions of each of the following:...Ch. 47.2 - Prob. 4CCh. 47.3 - Prob. 6LOCh. 47.3 - Prob. 7LOCh. 47.3 - Prob. 1CCh. 47.3 - Prob. 2CCh. 47.3 - Prob. 3CCh. 47.4 - Contrast basal metabolic rate with total metabolic...Ch. 47.4 - Prob. 9LOCh. 47.4 - Prob. 10LOCh. 47.4 - Prob. 1CCh. 47.4 - Prob. 2CCh. 47.4 - How do melanocortins, neuropeptides Y (NPY),...Ch. 47 - Test Your Understanding 1 Teeth adapted for...Ch. 47 - Prob. 2TYUCh. 47 - Prob. 3TYUCh. 47 - Test Your Understanding 4 Arrange the following...Ch. 47 - Test Your Understanding 5 Which sequence most...Ch. 47 - Prob. 6TYUCh. 47 - Lipids are transported from the intestine to the...Ch. 47 - Test Your Understanding 8 Most vitamins are (a)...Ch. 47 - Prob. 9TYUCh. 47 - Prob. 10TYUCh. 47 - Prob. 11TYUCh. 47 - Prob. 12TYUCh. 47 - Prob. 13TYUCh. 47 - Prob. 14TYUCh. 47 - Prob. 15TYU
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- 1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids? 2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following: Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng Final recovery volume: 0.50 mL Volume plated: 0.25 mL Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forward
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