1. PLATE NORMAL L WITHOUT PLASIMID +DMA -DNA --DAMP +TH-REASH +AMP NORMAL ALUWTH WITHOUT PLASIMID NO AFTIBOTIC RESITE INDUSIN RESISTANCE WIT PROSMID WITHOUT PLASMID AMP +AMP 5. -DNA +-AMP +AMP 4. PLATE +AMP. +IAMP -DNA -DNA/+AMP +DNA/+AMP +DNA/+AMP/+IPTG • No plasmid • No plasmid • Plasmid Plasmid No ampicillin • Ampicillin • Ampicillin • Ampicillin • No IPTG • No IPTG • No IPTG • IPTG
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
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