400 bp Hae III 380 bp Bam HI Eco RI 150 bp Hind II Plasmid DNA 2000 bp 250 bp 500 bp Eco RI 320 bp Eco RV a. Look up the restriction enzyme and indicate whether the restriction enzyme will produce a blunt or staggered cut. (1 mark) Note: Type either Blunt or Staggered Restriction enzyme Type of cut (Blunt vs Staggered) Eco RI b. Based on the plasmid map, list the size of fragments produced when the following restriction enzymes are used. Note: to answer correctly you must order the fragments from smallest to largest and separate each with a comma. There should be no spaces between numbers and commas. e.g. 400,500,530 Restriction Enzyme Eco RI and Hind II Eco RV and Bam HI Hae III, Eco RI and Bam HI Fragment size

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Solve the problem clearly and thoroughly, using the image for all relevant information. Once done, double-check the solution to ensure accuracy. On occasions I receive incorrect answers!. Please by considering that, check your working out and then finalise!.

400 bp
Hae III
380 bp
Bam HI
Eco RI
150 bp
Hind II
Plasmid DNA
2000 bp
250 bp
500 bp
Eco RI
320 bp
Eco RV
a. Look up the restriction enzyme and indicate whether the restriction enzyme will produce a blunt or staggered cut. (1 mark)
Note: Type either Blunt or Staggered
Restriction enzyme
Type of cut (Blunt vs Staggered)
Eco RI
b. Based on the plasmid map, list the size of fragments produced when the following restriction enzymes are used.
Note: to answer correctly you must order the fragments from smallest to largest and separate each with a comma. There should be no spaces between numbers and commas. e.g.
400,500,530
Restriction Enzyme
Eco RI and Hind II
Eco RV and Bam HI
Hae III, Eco RI and Bam HI
Fragment size
Transcribed Image Text:400 bp Hae III 380 bp Bam HI Eco RI 150 bp Hind II Plasmid DNA 2000 bp 250 bp 500 bp Eco RI 320 bp Eco RV a. Look up the restriction enzyme and indicate whether the restriction enzyme will produce a blunt or staggered cut. (1 mark) Note: Type either Blunt or Staggered Restriction enzyme Type of cut (Blunt vs Staggered) Eco RI b. Based on the plasmid map, list the size of fragments produced when the following restriction enzymes are used. Note: to answer correctly you must order the fragments from smallest to largest and separate each with a comma. There should be no spaces between numbers and commas. e.g. 400,500,530 Restriction Enzyme Eco RI and Hind II Eco RV and Bam HI Hae III, Eco RI and Bam HI Fragment size
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