Assuming the line is not perfect, what are some sources of error? If you state ‘human error’ be specific as to what the human did to cause error.
Assuming the line is not perfect, what are some sources of error? If you state ‘human error’ be specific as to what the human did to cause error.
With the help of Spectrophotometric analysis , we are able to calculate the concentration of a substance by measuring its absorbance value. Beer lamberts law tells us that Absorbance is directly proportional to the concentration of the analyte whose absorbance we measured. Hence a standard plot with Absorbance and Concentration of analyte as Y and X axis respectively can be obtained by measuring the absorbance of known concentrations of the analyte using spectrophotometer and then plotting the graph.
Once we plot the points , then we draw a best fit line i.e. a line that is the closest to all the points , this line is called the regression line (the blue dotted line in question) . Then we obtain the equation of the line which would be in the form ' y= mx +c'. Here 'y' is absorbance, 'm' is slope of the line, 'x' is concentration of analyte and 'c' is Y-intercept. We can calculate the concentration of the analyte in an unknown sample by simply measuring the absorbance value, substituting it as 'y' in equation and then solving for 'x' .
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