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1. PLATE NORMAL L WITHOUT PLASIMID +DMA -DNA --DAMP +TH-REASH +AMP NORMAL ALUWTH WITHOUT PLASIMID NO AFTIBOTIC RESITE INDUSIN RESISTANCE WIT PROSMID WITHOUT PLASMID AMP +AMP 5. -DNA +-AMP +AMP 4. PLATE +AMP. +IAMP -DNA -DNA/+AMP +DNA/+AMP +DNA/+AMP/+IPTG • No plasmid • No plasmid • Plasmid Plasmid No ampicillin • Ampicillin • Ampicillin • Ampicillin • No IPTG • No IPTG • No IPTG • IPTG

1. PLATE NORMAL L WITHOUT PLASIMID +DMA -DNA --DAMP +TH-REASH +AMP NORMAL ALUWTH WITHOUT PLASIMID NO AFTIBOTIC RESITE INDUSIN RESISTANCE WIT PROSMID WITHOUT PLASMID AMP +AMP 5. -DNA +-AMP +AMP 4. PLATE +AMP. +IAMP -DNA -DNA/+AMP +DNA/+AMP +DNA/+AMP/+IPTG • No plasmid • No plasmid • Plasmid Plasmid No ampicillin • Ampicillin • Ampicillin • Ampicillin • No IPTG • No IPTG • No IPTG • IPTG

Biology: The Unity and Diversity of Life (MindTap Course List)
Biology: The Unity and Diversity of Life (MindTap Course List)
14th Edition
ISBN:9781305073951
Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Chapter20: Viruses, Bacteria, And Archaea
Section: Chapter Questions
Problem 6SQ
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a) Calculating Transformation Efficiency

For the +DNA/+Amp/+IPTG plate, record the following: 

Number of transformants (colonies): _________________

Nanograms of plasmid DNA added: 50 ng 

Final recovery volume: 0.50 mL 

Volume plated: 0.25 mL 

Transformation efficiency equation:

Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)

b) Using the equation above, calculate the transformation efficiency.

c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?      

1. PLATE NORMAL L WITHOUT PLASIMID +DMA -DNA --DAMP +TH-REASH +AMP NORMAL ALUWTH WITHOUT PLASIMID NO AFTIBOTIC RESITE INDUSIN RESISTANCE WIT PROSMID WITHOUT PLASMID AMP +AMP 5. -DNA +-AMP +AMP 4. PLATE +AMP. +IAMP
Transcribed Image Text:1. PLATE NORMAL L WITHOUT PLASIMID +DMA -DNA --DAMP +TH-REASH +AMP NORMAL ALUWTH WITHOUT PLASIMID NO AFTIBOTIC RESITE INDUSIN RESISTANCE WIT PROSMID WITHOUT PLASMID AMP +AMP 5. -DNA +-AMP +AMP 4. PLATE +AMP. +IAMP
-DNA -DNA/+AMP +DNA/+AMP +DNA/+AMP/+IPTG • No plasmid • No plasmid • Plasmid Plasmid No ampicillin • Ampicillin • Ampicillin • Ampicillin • No IPTG • No IPTG • No IPTG • IPTG
Transcribed Image Text:-DNA -DNA/+AMP +DNA/+AMP +DNA/+AMP/+IPTG • No plasmid • No plasmid • Plasmid Plasmid No ampicillin • Ampicillin • Ampicillin • Ampicillin • No IPTG • No IPTG • No IPTG • IPTG
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