Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 33.4, Problem 2.2RIA
Summary Introduction
The major cells of the adaptive immune system are lymphocytes that divided into innate lymphoid cells, B cells, and T cells. A type of lymphocyte that plays an essential role in the immune system and matures in the thymus gland is called T cell. It can be distinguished from another kind of lymphocytes through the presence of T cell receptor on the surface of the cell.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 33 Solutions
Prescott's Microbiology
Ch. 33.1 - Prob. 1RIACh. 33.1 - Compare and contrast the adaptive and innate...Ch. 33.2 - Why is the skin such a good first line of defense...Ch. 33.2 - Prob. 2RIACh. 33.2 - Prob. 3RIACh. 33.3 - What strategies could a Gram-negative bacterium...Ch. 33.3 - Prob. 1.1RIACh. 33.3 - Prob. 1.2RIACh. 33.3 - Prob. 1.3RIACh. 33.3 - Prob. 1.4RIA
Ch. 33.3 - Prob. 1.5RIACh. 33.3 - Prob. 2.1RIACh. 33.3 - Prob. 2.2RIACh. 33.3 - How might acute-phase reactants assist in pathogen...Ch. 33.4 - For what does the dendritic cell use its cellular...Ch. 33.4 - Prob. 2MICh. 33.4 - Describe the structure and function of each of the...Ch. 33.4 - Prob. 1.2RIACh. 33.4 - Prob. 1.3RIACh. 33.4 - What is a plasma cell?Ch. 33.4 - Prob. 2.2RIACh. 33.4 - Discuss the role of NK cells in protecting the...Ch. 33.4 - Prob. 3.1RIACh. 33.4 - Injury to the spleen can lead to its removal. What...Ch. 33.4 - Describe SALT and MALT functions in innate...Ch. 33.5 - Prob. 1RIACh. 33.5 - Prob. 2RIACh. 33.5 - How do macrophages and dendritic cells become...Ch. 33.6 - Prob. 1MICh. 33.6 - Prob. 1RIACh. 33.6 - How does chronic inflammation differ from acute...Ch. 33 - Some pathogens invade cells; others invade tissue...Ch. 33 - How might the various antimicrobial chemical...Ch. 33 - How might a scientist use selective gene knockouts...Ch. 33 - Conservation of resources is a common theme in...
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