Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 31, Problem 15P
Interpretation Introduction
Interpretation :
The indicated mechanism needs to be explained.
Interpretation :
HtrA proteins and their orthologues signify the vital class of the heat-shock-induced serine proteases plus chaperones shielding the structure of protein. They are stated in both eukaryotic and prokaryotic species, comprising humans and plants.
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Sesamin is one of the major lignans found in sesame oil. Sesamin-metabolic pathways remain uncharacterized at both the enzyme and gene levels. However, one microorganism showed significant sesamin-degrading activity and was identified as Sinomonas sp. no. 22. A sesamin- metabolizing enzyme named SesA was purified from this strain and characterized. Explain how sesamin methylene transferase was identified and characterized and explain the mechanism. Based on your knowledge, what other assays could be run to characterize this enzyme?
M-CSA Mechanism and Catalytic Site Atlas (ebi.ac.uk)
(ii)
Acyl Carrier Protein S-acetyltransferase (EC 2.3.1.38) is a transferase enzyme that
catalyzes the first biosynthetic pathway for fatty acid synthase. It transfers the acyl
group (CH3CO) first from coenzyme A to a cysteine residue in the active site. This is
similar to what happens in Chymotrypsin, however utilizing a sulfur instead of an
oxygen. The acyl group is then transferred to the molecule ACP. Provide the enzyme-
catalyzed mechanism for the reaction below, making sure to identify the roles of all key
amino acids:
i
H3C
SCOA
acetyl COA
enzyme
+ HS
i
H3C
SACP
acetyl ACP
Predict the effect of each of the following amino acid substitutions on the KM and kcat of the enzyme-catalyzed reactions
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