BIOCHEMISTRY
9th Edition
ISBN: 2818440090622
Author: BERG
Publisher: MAC HIGHER
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Chapter 29, Problem 16P
Interpretation Introduction
Interpretation:
The maximum number of base pairs that could be broken per ATP hydrolyzed by a helicase operating under standard conditions needs to be estimated.
Concept introduction:
In replication, the two strands of double-stranded DNA molecule needs to be separated from each other for a sort local stretch. This needs helicases with association with ATP hydrolysis.
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Specific rotation is a measure of a solution's capacity to rotate circularly polarized light. The unfolding of the a helix of a
polypeptide to a random conformation is accompanied by a large decrease in specific rotation.
Polyglutamate, a polypeptide made up of only 1-Glu residues, has the a helix conformation at pH 3. When researchers raise the
pH to 7, there is a large decrease in the specific rotation of the solution. Similarly, polylysine (1.-Lys residues) is an a helix at pH
10, but when researchers lower the pH to 7 the specific rotation also decreases, as shown in the graph.
a Helix
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a Helix
Random
conformation
Poly(Lys)
Random conformation
T
+
°
2
4
6 В
10 12 14
PH
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Increasing the pH of a polyglutamate solution from 6 to 7 causes the carboxyl group of each glutamate residue
Comed Artwer
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From the reaction data below, determine whether the reaction is first order or second order and calculate the rate constant.
Time (s)
0
Reactant (mM)
6.2
1
3.1
2
2.1
3
1.6
4
1.3
5
1.1
Only a plot of 1/[reactant] versus t gives a straight line, so the reaction is
0.150
mM-1 s-1
. S
second order
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From the reaction data below, determine whether the reaction is first order or second order and calculate the rate constant.
Time (s)
0
Reactant (mM)
5.4
1
4.6
2
3.9
3
3.2
4
2.7
5
2.3
Only a plot of In[reactant] versus t gives a straight line, so the reaction is
s-1.
. The negative of the slope, k, is
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