(a) Interpretation: The purpose of the control plate to be exposed only to water needs to be explained. Concept introduction: Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

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Answer 1

Question

Chapter 29, Problem 20P

Interpretation Introduction

(a)

Interpretation:

The purpose of the control plate to be exposed only to water needs to be explained.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Interpretation Introduction

(b)

Interpretation:

The use of known mutant in a given experiment is to be described.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Interpretation Introduction

(c)

Interpretation:

The results obtained from the experimental compound needs to be interpreted.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Interpretation Introduction

(d)

Interpretation:

Unknown liver compound used in sample D in a given experiment is to be described.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

Question

Chapter 29, Problem 20P

Interpretation Introduction

(a)

Interpretation:

The purpose of the control plate to be exposed only to water needs to be explained.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Interpretation Introduction

(b)

Interpretation:

The use of known mutant in a given experiment is to be described.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Interpretation Introduction

(c)

Interpretation:

The results obtained from the experimental compound needs to be interpreted.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Interpretation Introduction

(d)

Interpretation:

Unknown liver compound used in sample D in a given experiment is to be described.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

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Answer 3

Question

Chapter 29, Problem 20P

Interpretation Introduction

(a)

Interpretation:

The purpose of the control plate to be exposed only to water needs to be explained.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Interpretation Introduction

(b)

Interpretation:

The use of known mutant in a given experiment is to be described.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Interpretation Introduction

(c)

Interpretation:

The results obtained from the experimental compound needs to be interpreted.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Interpretation Introduction

(d)

Interpretation:

Unknown liver compound used in sample D in a given experiment is to be described.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

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Answer 4

Question

Chapter 29, Problem 20P

Interpretation Introduction

(a)

Interpretation:

The purpose of the control plate to be exposed only to water needs to be explained.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Interpretation Introduction

(b)

Interpretation:

The use of known mutant in a given experiment is to be described.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Interpretation Introduction

(c)

Interpretation:

The results obtained from the experimental compound needs to be interpreted.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Interpretation Introduction

(d)

Interpretation:

Unknown liver compound used in sample D in a given experiment is to be described.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

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Answer 5

Chapter 29, Problem 20P

Interpretation Introduction

(a)

Interpretation:

The purpose of the control plate to be exposed only to water needs to be explained.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Interpretation Introduction

(b)

Interpretation:

The use of known mutant in a given experiment is to be described.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Interpretation Introduction

(c)

Interpretation:

The results obtained from the experimental compound needs to be interpreted.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Interpretation Introduction

(d)

Interpretation:

Unknown liver compound used in sample D in a given experiment is to be described.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Answer 6

Chapter 29, Problem 20P

Interpretation Introduction

(a)

Interpretation:

The purpose of the control plate to be exposed only to water needs to be explained.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Answer 7

Chapter 29, Problem 20P

Interpretation Introduction

(a)

Interpretation:

The purpose of the control plate to be exposed only to water needs to be explained.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The use of known mutant in a given experiment is to be described.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The use of known mutant in a given experiment is to be described.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Answer 10

Interpretation Introduction

(c)

Interpretation:

The results obtained from the experimental compound needs to be interpreted.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Answer 11

Interpretation Introduction

(c)

Interpretation:

The results obtained from the experimental compound needs to be interpreted.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Answer 12

Interpretation Introduction

(d)

Interpretation:

Unknown liver compound used in sample D in a given experiment is to be described.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Answer 13

Interpretation Introduction

(d)

Interpretation:

Unknown liver compound used in sample D in a given experiment is to be described.

Concept introduction:

Ames test is used to check if the given chemical is a mutation causing agent or not i.e. it can cause mutation in the DNA of the given organism or not. This test uses bacteria that lacks histidine forming machinery.

Answer 14

Expert Solution & Answer

Answer 15

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Answer 16

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Answer 17

Ch. 29 - Prob. 1P Ch. 29 - Prob. 2P Ch. 29 - Prob. 3P Ch. 29 - Prob. 4P Ch. 29 - Prob. 5P Ch. 29 - Prob. 6P Ch. 29 - Prob. 7P Ch. 29 - Prob. 8P Ch. 29 - Prob. 9P Ch. 29 - Prob. 10P

Solution Summary: The author explains the purpose of the control plate to be exposed only to water. Ames test is used to check if the chemical is a mutation causing agent or not.

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Chapter 29 Solutions