(a) Interpretation: Auto radiographic pattern of unidirectional replication is to be sketched. Concept introduction: Replication is the process of DNA to copy itself with the help of different enzymes. Replication starts from a single point known as origin of replication. If from the starting point it proceeds in one direction, it will be known as unidirectional. If proceeds in both directions known as bidirectional replication.

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Answer 1

Question

Chapter 29, Problem 12P

Interpretation Introduction

(a)

Interpretation:

Auto radiographic pattern of unidirectional replication is to be sketched.

Concept introduction:

Replication is the process of DNA to copy itself with the help of different enzymes. Replication starts from a single point known as origin of replication. If from the starting point it proceeds in one direction, it will be known as unidirectional. If proceeds in both directions known as bidirectional replication.

Interpretation Introduction

(b)

Interpretation:

Auto radiographic pattern of bidirectional replication is to be sketched.

Concept introduction:

Replication is the process of DNA to copy itself with the help of different enzymes. Replication starts from a single point known as origin of replication. If from the starting point it proceeds in one direction it will be known as unidirectional and if proceeds in both directions known as bidirectional replication.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

Question

Chapter 29, Problem 12P

Interpretation Introduction

(a)

Interpretation:

Auto radiographic pattern of unidirectional replication is to be sketched.

Concept introduction:

Replication is the process of DNA to copy itself with the help of different enzymes. Replication starts from a single point known as origin of replication. If from the starting point it proceeds in one direction, it will be known as unidirectional. If proceeds in both directions known as bidirectional replication.

Interpretation Introduction

(b)

Interpretation:

Auto radiographic pattern of bidirectional replication is to be sketched.

Concept introduction:

Replication is the process of DNA to copy itself with the help of different enzymes. Replication starts from a single point known as origin of replication. If from the starting point it proceeds in one direction it will be known as unidirectional and if proceeds in both directions known as bidirectional replication.

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Answer 3

Question

Chapter 29, Problem 12P

Interpretation Introduction

(a)

Interpretation:

Auto radiographic pattern of unidirectional replication is to be sketched.

Concept introduction:

Replication is the process of DNA to copy itself with the help of different enzymes. Replication starts from a single point known as origin of replication. If from the starting point it proceeds in one direction, it will be known as unidirectional. If proceeds in both directions known as bidirectional replication.

Interpretation Introduction

(b)

Interpretation:

Auto radiographic pattern of bidirectional replication is to be sketched.

Concept introduction:

Replication is the process of DNA to copy itself with the help of different enzymes. Replication starts from a single point known as origin of replication. If from the starting point it proceeds in one direction it will be known as unidirectional and if proceeds in both directions known as bidirectional replication.

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Answer 4

Question

Chapter 29, Problem 12P

Interpretation Introduction

(a)

Interpretation:

Auto radiographic pattern of unidirectional replication is to be sketched.

Concept introduction:

Replication is the process of DNA to copy itself with the help of different enzymes. Replication starts from a single point known as origin of replication. If from the starting point it proceeds in one direction, it will be known as unidirectional. If proceeds in both directions known as bidirectional replication.

Interpretation Introduction

(b)

Interpretation:

Auto radiographic pattern of bidirectional replication is to be sketched.

Concept introduction:

Replication is the process of DNA to copy itself with the help of different enzymes. Replication starts from a single point known as origin of replication. If from the starting point it proceeds in one direction it will be known as unidirectional and if proceeds in both directions known as bidirectional replication.

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Answer 5

Chapter 29, Problem 12P

Interpretation Introduction

(a)

Interpretation:

Auto radiographic pattern of unidirectional replication is to be sketched.

Concept introduction:

Replication is the process of DNA to copy itself with the help of different enzymes. Replication starts from a single point known as origin of replication. If from the starting point it proceeds in one direction, it will be known as unidirectional. If proceeds in both directions known as bidirectional replication.

Interpretation Introduction

(b)

Interpretation:

Auto radiographic pattern of bidirectional replication is to be sketched.

Concept introduction:

Replication is the process of DNA to copy itself with the help of different enzymes. Replication starts from a single point known as origin of replication. If from the starting point it proceeds in one direction it will be known as unidirectional and if proceeds in both directions known as bidirectional replication.

Answer 6

Chapter 29, Problem 12P

Interpretation Introduction

(a)

Interpretation:

Auto radiographic pattern of unidirectional replication is to be sketched.

Concept introduction:

Replication is the process of DNA to copy itself with the help of different enzymes. Replication starts from a single point known as origin of replication. If from the starting point it proceeds in one direction, it will be known as unidirectional. If proceeds in both directions known as bidirectional replication.

Answer 7

Chapter 29, Problem 12P

Interpretation Introduction

(a)

Interpretation:

Auto radiographic pattern of unidirectional replication is to be sketched.

Concept introduction:

Replication is the process of DNA to copy itself with the help of different enzymes. Replication starts from a single point known as origin of replication. If from the starting point it proceeds in one direction, it will be known as unidirectional. If proceeds in both directions known as bidirectional replication.

Answer 8

Interpretation Introduction

(b)

Interpretation:

Auto radiographic pattern of bidirectional replication is to be sketched.

Concept introduction:

Replication is the process of DNA to copy itself with the help of different enzymes. Replication starts from a single point known as origin of replication. If from the starting point it proceeds in one direction it will be known as unidirectional and if proceeds in both directions known as bidirectional replication.

Answer 9

Interpretation Introduction

(b)

Interpretation:

Auto radiographic pattern of bidirectional replication is to be sketched.

Concept introduction:

Replication is the process of DNA to copy itself with the help of different enzymes. Replication starts from a single point known as origin of replication. If from the starting point it proceeds in one direction it will be known as unidirectional and if proceeds in both directions known as bidirectional replication.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 29 - Prob. 1P Ch. 29 - Prob. 2P Ch. 29 - Prob. 3P Ch. 29 - Prob. 4P Ch. 29 - Prob. 5P Ch. 29 - Prob. 6P Ch. 29 - Prob. 7P Ch. 29 - Prob. 8P Ch. 29 - Prob. 9P Ch. 29 - Prob. 10P

Solution Summary: The author illustrates the auto radiographic pattern of unidirectional and bidirectional replication.

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