Chapter 26, Problem 26.33EP

(a)

Interpretation Introduction

Interpretation: To determine whether oxaloacetate and $\text{α-ketoglutarate}$could function as the two reactants in a transamination reaction or not.

Concept introduction: Transamination reaction is a biochemical reaction that involves the transfer of an amino group. In transamination reaction exchange of an amino group from an $\text{α-amino}$acid with a keto group of an $\text{α-keto}$acid. There occurs no net loss or gain of amino acid in transamination reaction. The general reaction to illustrate transamination is as follows:

The general structure of an amino acid is:

Here, $\text{R}$is the side chain which is different for a different amino acid.

An acid containing both carbonyl and carboxyl functional group is known as a keto acid. A general representation of a keto acid is:

Answer to Problem 26.33EP

No, oxaloacetate and $\text{α-ketoglutarate}$cannot function as the reactants in a transamination reaction.

Explanation of Solution

$\text{α-ketoglutarate}$ is keto acid and its structure is:

Oxaloacetate is a keto acid and its structure is:

The two reactants in transamination reaction are a keto acid and an amino acid. Both oxaloacetate and $\text{α-ketoglutarate}$ are keto acids thus they cannot function as reactants in a transamination reaction. For a transamination reaction to take place there must be one amino acid present along with a keto acid.

(b)

Interpretation Introduction

Interpretation: To determine whether glutamate and oxaloacetate could function as the two reactants in a transamination reaction or not.

Concept introduction: Transamination reaction is a biochemical reaction that involves the transfer of an amino group. In transamination reaction exchange of an amino group from an $\text{α-amino}$acid with a keto group of an $\text{α-keto}$acid. There occurs no net loss or gain of amino acid in transamination reaction. The general reaction to illustrate transamination is as follows:

The general structure of an amino acid is:

Here, $\text{R}$is the side chain which is different for a different amino acid.

An acid containing both carbonyl and carboxyl functional group is known as a keto acid. A general representation of a keto acid is:

Answer to Problem 26.33EP

Yes, glutamate and oxaloacetate can function as the reactants in a transamination reaction.

Explanation of Solution

Glutamate is an amino acid and its structure is:

Oxaloacetate is a keto acid and its structure is:

Transamination reaction involves the exchange of an amino group from an $\text{α-amino}$acid with a keto group of an $\text{α-keto}$acid. The two reactants in transamination reaction are a keto acid and an amino acid. Since oxaloacetate is a keto acid and glutamate is an amino acid they can function as reactants in a transamination reaction.

(c)

Interpretation Introduction

Interpretation: To determine whether glutarate and glutamate could function as the two reactants in a transamination reaction or not.

Concept introduction: Transamination reaction is a biochemical reaction that involves the transfer of an amino group. In transamination reaction exchange of an amino group from an $\text{α-amino}$acid with a keto group of an $\text{α-keto}$acid. There occurs no net loss or gain of amino acid in transamination reaction. The general reaction to illustrate transamination is as follows:

The general structure of an amino acid is:

Here, $\text{R}$is the side chain which is different for a different amino acid.

An acid containing both carbonyl and carboxyl functional group is known as a keto acid. A general representation of a keto acid is:

Answer to Problem 26.33EP

No, glutarate and glutamate cannot function as the reactants in a transamination reaction.

Explanation of Solution

Glutarate is a diacid and its structure is:

Glutamate is an amino acid and its structure is:

The two reactants in transamination reaction are a keto acid and an amino acid. Glutamate is an amino acid but glutarate is not a keto acid. For a transamination reaction to take place there must be one keto acid present along with an amino acid. Thus, glutarate and glutamate cannot function as the reactants in a transamination reaction.

(d)

Interpretation Introduction

Interpretation: To determine whether oxaloacetate and succinate could function as the two reactants in a transamination reaction or not.

Concept introduction: Transamination reaction is a biochemical reaction that involves the transfer of an amino group. In transamination reaction exchange of an amino group from an $\text{α-amino}$acid with a keto group of an $\text{α-keto}$acid. There occurs no net loss or gain of amino acid in transamination reaction.

The general reaction to illustrate transamination is as follows:

The general structure of an amino acid is:

Here, $\text{R}$is the side chain which is different for a different amino acid.

An acid containing both carbonyl and carboxyl functional group is known as a keto acid. A general representation of a keto acid is:

Answer to Problem 26.33EP

No, oxaloacetate and succinate cannot function as the reactants in a transamination reaction.

Explanation of Solution

Oxaloacetate is a keto acid and its structure is:

Succinate is a diacid acid and its structure is:

The two reactants in transamination reaction are a keto acid and an amino acid. Oxaloacetate is keto acid but succinate is not an amino acid. For a transamination reaction to take place there must be one amino acid present along with a keto acid. Thus, oxaloacetate and succinate cannot function as the reactants in a transamination reaction.