EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Chapter 21, Problem 4FIB
Summary Introduction
Introduction:
Disseminated intravascular coagulation involves blood coagulation throughout the body system by blocking the small blood vessels. This results in multiorgan failure and excessive bleeding from multiple locations, such as rectum or vagina. Disseminated intravascular coagulation is caused by degradation of microthrombi, consumption of platelets, reduction of coagulation factors, and reduction of fibrinogen in the body.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 21 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 21 - Bacteria infecting the mouth can enter the blood...Ch. 21 - Prob. 1CCSCh. 21 - Nightmare on the Island Peggy loves her time on...Ch. 21 - Prob. 2TMWCh. 21 - Prob. 3CCSCh. 21 - Prob. 4CCSCh. 21 - Prob. 3TMWCh. 21 - Prob. 1EDCSCh. 21 - Why can people who avoid cats get infected by...Ch. 21 - Prob. 5CCS
Ch. 21 - Prob. 2EDCSCh. 21 - Prob. 1MCCh. 21 - Prob. 2MCCh. 21 - Prob. 3MCCh. 21 - Prob. 4MCCh. 21 - Prob. 5MCCh. 21 - Prob. 6MCCh. 21 - Prob. 7MCCh. 21 - How is septicemia introduced into the body? a....Ch. 21 - Prob. 9MCCh. 21 - Prob. 10MCCh. 21 - A cardiologist examines a patient with history of...Ch. 21 - Prob. 12MCCh. 21 - Prob. 13MCCh. 21 - Which is spread from person to person? a....Ch. 21 - Which of the following statements is false? a. A...Ch. 21 - Prob. 16MCCh. 21 - Prob. 17MCCh. 21 - Prob. 18MCCh. 21 - Prob. 19MCCh. 21 - Prob. 20MCCh. 21 - Prob. 21MCCh. 21 - Prob. 22MCCh. 21 - Prob. 23MCCh. 21 - Prob. 24MCCh. 21 - Prob. 25MCCh. 21 - Prob. 1MCh. 21 - Prob. 2MCh. 21 - Prob. 1FIBCh. 21 - Prob. 2FIBCh. 21 - The infective stage of Plasmodium in humans is the...Ch. 21 - Prob. 4FIBCh. 21 - Prob. 5FIBCh. 21 - The three parts of the life cycle of Plasmodium...Ch. 21 - Prob. 7FIBCh. 21 - Prob. 8FIBCh. 21 - Prob. 9FIBCh. 21 - Prob. 10FIBCh. 21 - On the figure of the life cycle of Plasmodium,...Ch. 21 - Prob. 2VICh. 21 - List the three layers of the heart in order from...Ch. 21 - Prob. 2SACh. 21 - Prob. 3SACh. 21 - Prob. 4SACh. 21 - Define occult septicemia.Ch. 21 - Prob. 6SACh. 21 - Prob. 7SACh. 21 - Prob. 8SACh. 21 - Prob. 9SACh. 21 - If 13 million people die annually from malaria,...Ch. 21 - Prob. 11SACh. 21 - Prob. 12SACh. 21 - Prob. 13SACh. 21 - Why do geneticists say there is a single species...Ch. 21 - Explain why poor hygiene actually protects against...Ch. 21 - A blood bank refused to accept blood from a...Ch. 21 - An epidemiologist notices a statistical difference...Ch. 21 - Prob. 3CTCh. 21 - Compare and contrast the life cycles of...Ch. 21 - Explain how each of the following could lead to...Ch. 21 - Discuss why sickle-cell trait is advantageous to...Ch. 21 - Prob. 7CTCh. 21 - Prob. 8CTCh. 21 - Prob. 9CTCh. 21 - Most cases of tularemia in the United States occur...Ch. 21 - Prob. 11CTCh. 21 - Suppose scientists developed a vaccine for dengue...Ch. 21 - Prob. 13CTCh. 21 - Prob. 14CTCh. 21 - Prob. 1CM
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