EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Chapter 21, Problem 5FIB
Summary Introduction
Introduction:
Brucella is Gram-negative, nonmotile, aerobic coccobacillus that lack capsule. The species Brucella melitensis of this bacterium causes brucellosis in humans which is transmitted by affected animals. The signs and symptoms of the brucellosis are characterized by fluctuating fever which spike usually in afternoon, chills, headache, sweating, muscle pain, and weight loss.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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In oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be
purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the
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OTMPD is electron acceptor
O cytochrome c is the electron source
oxygen is terminal electron acceptor
OH2 produced is electron donor
Chapter 21 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 21 - Bacteria infecting the mouth can enter the blood...Ch. 21 - Prob. 1CCSCh. 21 - Nightmare on the Island Peggy loves her time on...Ch. 21 - Prob. 2TMWCh. 21 - Prob. 3CCSCh. 21 - Prob. 4CCSCh. 21 - Prob. 3TMWCh. 21 - Prob. 1EDCSCh. 21 - Why can people who avoid cats get infected by...Ch. 21 - Prob. 5CCS
Ch. 21 - Prob. 2EDCSCh. 21 - Prob. 1MCCh. 21 - Prob. 2MCCh. 21 - Prob. 3MCCh. 21 - Prob. 4MCCh. 21 - Prob. 5MCCh. 21 - Prob. 6MCCh. 21 - Prob. 7MCCh. 21 - How is septicemia introduced into the body? a....Ch. 21 - Prob. 9MCCh. 21 - Prob. 10MCCh. 21 - A cardiologist examines a patient with history of...Ch. 21 - Prob. 12MCCh. 21 - Prob. 13MCCh. 21 - Which is spread from person to person? a....Ch. 21 - Which of the following statements is false? a. A...Ch. 21 - Prob. 16MCCh. 21 - Prob. 17MCCh. 21 - Prob. 18MCCh. 21 - Prob. 19MCCh. 21 - Prob. 20MCCh. 21 - Prob. 21MCCh. 21 - Prob. 22MCCh. 21 - Prob. 23MCCh. 21 - Prob. 24MCCh. 21 - Prob. 25MCCh. 21 - Prob. 1MCh. 21 - Prob. 2MCh. 21 - Prob. 1FIBCh. 21 - Prob. 2FIBCh. 21 - The infective stage of Plasmodium in humans is the...Ch. 21 - Prob. 4FIBCh. 21 - Prob. 5FIBCh. 21 - The three parts of the life cycle of Plasmodium...Ch. 21 - Prob. 7FIBCh. 21 - Prob. 8FIBCh. 21 - Prob. 9FIBCh. 21 - Prob. 10FIBCh. 21 - On the figure of the life cycle of Plasmodium,...Ch. 21 - Prob. 2VICh. 21 - List the three layers of the heart in order from...Ch. 21 - Prob. 2SACh. 21 - Prob. 3SACh. 21 - Prob. 4SACh. 21 - Define occult septicemia.Ch. 21 - Prob. 6SACh. 21 - Prob. 7SACh. 21 - Prob. 8SACh. 21 - Prob. 9SACh. 21 - If 13 million people die annually from malaria,...Ch. 21 - Prob. 11SACh. 21 - Prob. 12SACh. 21 - Prob. 13SACh. 21 - Why do geneticists say there is a single species...Ch. 21 - Explain why poor hygiene actually protects against...Ch. 21 - A blood bank refused to accept blood from a...Ch. 21 - An epidemiologist notices a statistical difference...Ch. 21 - Prob. 3CTCh. 21 - Compare and contrast the life cycles of...Ch. 21 - Explain how each of the following could lead to...Ch. 21 - Discuss why sickle-cell trait is advantageous to...Ch. 21 - Prob. 7CTCh. 21 - Prob. 8CTCh. 21 - Prob. 9CTCh. 21 - Most cases of tularemia in the United States occur...Ch. 21 - Prob. 11CTCh. 21 - Suppose scientists developed a vaccine for dengue...Ch. 21 - Prob. 13CTCh. 21 - Prob. 14CTCh. 21 - Prob. 1CM
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forward
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