The sequence of the initial protein from the given partially hydrolyzed peptide parts of an 18- amino acid polypeptide is to be determined. Concept introduction: Proteins are large molecules, which are constructed from the α- amino acids. The amino acids are joined by groups characteristic of amides in a stable protein. Since an amide is the least reactive acid derivative, it requires relatively extreme conditions to hydrolyze them. Thus to compete, hydrolysis of a protein requires strongly acidic condition and very high temperatures. The relative amounts of each type of amino acid in a protein can be determined by a complete hydrolysis of the protein. To determine the amino acid sequence of a protein, the Edman degradation method is used.

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Chapter 21, Problem 21.35P

Interpretation Introduction

Interpretation:

The sequence of the initial protein from the given partially hydrolyzed peptide parts of an 18- amino acid polypeptide is to be determined.

Concept introduction:

Proteins are large molecules, which are constructed from the α- amino acids. The amino acids are joined by groups characteristic of amides in a stable protein. Since an amide is the least reactive acid derivative, it requires relatively extreme conditions to hydrolyze them. Thus to compete, hydrolysis of a protein requires strongly acidic condition and very high temperatures. The relative amounts of each type of amino acid in a protein can be determined by a complete hydrolysis of the protein. To determine the amino acid sequence of a protein, the Edman degradation method is used.

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