Concept explainers
To determine: Whether the cloning of dogs using nuclei from cells of a pet will develop clones identical to the original pet.
Concept introduction:
Reproductive cloning refers to the production of genetically identical version of an existing organism. This is done through “somatic cell nuclear transfer”. The nucleus of an egg is replaced with nucleus of somatic body cell (differentiated cell) known as donor nucleus. This is implanted into surrogate mother where the embryo develops into so-called clone. The somatic cell used for this procedure is a differentiated body cell.
To determine: The ethical questions that arise in performing reproductive cloning.
Concept introduction:
Reproductive cloning refers to the production of genetically identical version of an existing organism. This is done through “somatic cell nuclear transfer”. The nucleus of an egg is replaced with nucleus of somatic body cell (differentiated cell) known as donor nucleus. This is implanted into surrogate mother where the embryo develops into so-called clone. The somatic cell used for this procedure is a differentiated body cell.
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CAMPBELL BIOLOGY-W/MOD.MASTERBIOLOGY
- What is your stand about humans being ‘cloned’? Explain.arrow_forwardAs shown , several medical agents are now commercially produced by genetically engineered microorganisms. Discuss the advantages and disadvantages of making these agents this way.arrow_forwardCloning vectors are not just limited to bacterial plasmids. Bacteriophages and M13 phage vectors are also commonly utilized in the cloning process. State any five (5) key criteria to be an effective cloning vector.arrow_forward
- The idea behind PCR-based diagnostics is that a very small number of microbial genomes in a patient sample can be multiplied by PCR and more easily detected by the clinical team managing the patient’s care. Also, genetic-based diagnostics are very useful for viral infections because we don’t have biochemical tests, etc. to distinguish one virus from another (remember, viruses are metabolically inactive). However, a lot of work goes into the development of these tests. For instance, PCR requires primers that are complementary to the viral genome that is being copied. If primers are complementary to the target genome, what must scientists know to design primers that bind to the viral genome to be copied? (I mean this to be a general question; don’t look up the details of designing primers)arrow_forwardAnswer each of the following questions correctly.(2-5 sentences only)(DO NOT USE THE EXAMPLE I PROVIDED FOR YOUR ANSWER( A. How cloning and expression of certain genes allows for massive production of the desired product.(Give an specific example, AGAIN PLEASE DO NOT USE THIS EXAMPLE AS YOUR EXAMPLE FOR MY QUESTION. YOU ARE NOT ALLOWED TO COPY AND PASTE MY EXAMPLE FOR YOUR ANSWER. PLEASE PROVIDE ANOTHER EXAMPLE .DO NOT USE MY EXAMPLE) For example: the cloning and expression of insulin in bacteria allows for the mass production of this necessary protein for use by diabetic patients.arrow_forwardCloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forward
- Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.arrow_forwardWhat Are Clones? Cloning is a general term used for whole organisms and DNA sequences. Define what we mean when we say we have a clone.arrow_forwardPCR is quick, efficient and easy to perform. However, there are some situations when cell-based cloning is preferred over PCR to amplify a DNA sequence. Mention two of them.arrow_forward
- fomP is responsible for the chemical transformation of microplastics into ultra-efficient insulation. You take an arctic seawater sample and extract the DNA. 1. First you need to locate the gene on the bacterial chromosome. What procedure(s) would you use to identify and locate the gene? Explain how it/theywork(s). 2. Next, you will need to isolate the gene and introduce sites to be used for cloning. What would you use to make many copies of this gene? What will you need? How does it work on a molecular level?arrow_forwardWhy does one need a vector to clone a DNAarrow_forwardCan someone tell me why the answer is that the spill contained transition inducing mutagens?arrow_forward
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