CAMPBELL BIOLOGY-W/MOD.MASTERBIOLOGY
CAMPBELL BIOLOGY-W/MOD.MASTERBIOLOGY
11th Edition
ISBN: 9780134683461
Author: Urry
Publisher: PEARSON
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Chapter 20, Problem 15TYU

The water in the Yellowstone National Park hot springs shown here is around 160°F (70°C). Biologists assumed that no species of organismscould live in water above about 130°F(55*C), so thev were surprised to find several species of bacteria there, now called thermophiles

15. SYNTHESIZE YOUR KNOWLEDGE

Chapter 20, Problem 15TYU, The water in the Yellowstone National Park hot springs shown here is around 160F (70C). Biologists

("heat-lovers"). You've learned in this chapter how an enzyme from one species, Thermus aquaticus, made feasible one of the most important DNA-based techniques used in labs today. ldentify the enzyme, and indicate the value of its being isolated from a thermophile. Suggest other reasons why enzymes from this bacterium (or other therrnophiles) might also be valuable.

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In one final experiment, you add all the necessary components and program the thermocycler to cycle under the following temperatures Denaturation 95 degrees, 30 seconds, Annealing 65 degrees, 30 seconds, Elongation 68 degrees 30 seconds  You find out that the third time's the charm, but now you have many small incomplete fragments instead. What happened and how did you fix it?
Knowing that for a bacterial colony to be able to grow it must produce product "3" AND "4", use the information in the image to describe which enzyme(s) are that are Non-Functional in Colony C? Please note error in enzyme description at bottom of image. X converts 1 into 2; Y converts 1 into 3; and z converts 2 into 4.
Which two enzyme curves would be generated from a themophilic bacteria that lives in a strongly acid environment? Please provide a supportive statement about why you picked each of the two curves you picked. Your answer should include information from the graphs. (a) 3 5 MA A 100 7 8 20 2 40 60 Temperature (°C) I 80 ➤ (b) 1 2 3 4 PH 5 6 ➤ 9 10
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