Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Textbook Question
Chapter 20.14, Problem 1CR
Would you expect to find the same types of microorganisms associated with black smoker and carbonate vent systems? Explain.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 20 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 20.1 - Prob. 1MQCh. 20.1 - Prob. 2MQCh. 20.1 - Prob. 3MQCh. 20.1 - How does a microbial population differ from a...Ch. 20.1 - Prob. 1CRCh. 20.2 - Prob. 1MQCh. 20.2 - What is a biogeochemical cycle? Give an example...Ch. 20.2 - Prob. 1CRCh. 20.3 - Prob. 1MQCh. 20.3 - Prob. 2MQ
Ch. 20.3 - Prob. 1CRCh. 20.4 - Prob. 1MQCh. 20.4 - Prob. 2MQCh. 20.4 - Prob. 3MQCh. 20.4 - Prob. 1CRCh. 20.5 - Prob. 1MQCh. 20.5 - Prob. 2MQCh. 20.5 - Prob. 1CRCh. 20.6 - Which phylum of Bacteria dominates bacterial...Ch. 20.6 - What factors govern the extent and type of...Ch. 20.6 - Prob. 3MQCh. 20.6 - In what soil horizon are microbial numbers and...Ch. 20.7 - Prob. 1MQCh. 20.7 - Prob. 2MQCh. 20.7 - Prob. 3MQCh. 20.7 - Prob. 1CRCh. 20.8 - Prob. 1MQCh. 20.8 - Prob. 2MQCh. 20.8 - Prob. 3MQCh. 20.8 - Prob. 1CRCh. 20.9 - What did the Deepwater Horizon spill tell us about...Ch. 20.9 - Prob. 2MQCh. 20.9 - Why is release of sulfide from oxygen minimum...Ch. 20.10 - How does Ostreococcus differ from Prochlorococcus?...Ch. 20.10 - How does the organism Prochlorococcus contribute...Ch. 20.10 - How does Roseobacter differ from Prochlorococcus?Ch. 20.10 - Prob. 1CRCh. 20.11 - What is proteorhodopsin and why is it so named?...Ch. 20.11 - Prob. 2MQCh. 20.11 - Prob. 3MQCh. 20.11 - Why are dilute culture media used for isolating...Ch. 20.11 - Prob. 1CRCh. 20.12 - Prob. 1MQCh. 20.12 - What molecular adaptations are found in...Ch. 20.12 - Prob. 1CRCh. 20.13 - Prob. 1MQCh. 20.13 - Prob. 2MQCh. 20.13 - What alternative sources of energy are suggested...Ch. 20.13 - Deep-sea sediments show decreasing nutrient levels...Ch. 20.14 - How does a warm hydrothermal vent differ from a...Ch. 20.14 - Prob. 2MQCh. 20.14 - Prob. 3MQCh. 20.14 - Would you expect to find the same types of...Ch. 20 - Prob. 1AQCh. 20 - Prob. 2AQCh. 20 - Global warming has been suggested to result in...
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have shipped your samples off for sequencing and are now waiting for the results. Out of curiosity (and maybe boredom...) you decide to test your culture for the Catalase and Oxidase enzymes. Upon testing your sample for catalase, you don't see any bubbles; however, you do see a color change to purple during the Oxidase test. What results can you conclude from this? O Catalase-/ Oxidase + O Catalase +/ Oxidase + Catalase + / Oxidase- O Catalase / Oxidase - O None of the abovearrow_forwardWhich of the following is not a strength of using 16S rRNA for phylogenetic analyses? OA. It's cheap OB. It's easy to do C. It can be used to identify all the way down to the strain level OD. Both A & B OE. None of the abovearrow_forwardWhy are molecular approaches important to the field of microbial taxonomy and phylogeny? Phylogenetic inferences based on molecular approaches provide the most robust analysis of microbial evolution currently available. It allows for the collection of a large and accurate dataset from many organisms Almost no fossil record was left by microbes when compared to plants and animals All of the above None of the abovearrow_forward
- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forward
- Which of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forwardAn unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forwardWhat is the most appropriate purpose of building a phylogenetic tree? They look awesome You can use a tree to compare morphological characteristics of organisms It can be used to establish and analyze evolutionary relationships between species All of the abovearrow_forward
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