Genetics: A Conceptual Approach
Genetics: A Conceptual Approach
6th Edition
ISBN: 9781319127121
Author: Pierce
Publisher: MAC HIGHER
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Chapter 19.6, Problem 42AQP

a.

Summary Introduction

To explain:

The result of double-stranded RNA (dsRNA) on gene expression.

Introduction:

Gene expression is provides the information from DNA to RNA to protein, it is process in which the information of gene is used in the synthesis of a functional gene product. It involve the two stage, transcription, where the production of mRNA is done by using RNA polymerase and the processing of the resulting mRNA molecule. The gfp is a green fluorescent protein, it used as reporter gene in the experiment to determine the gene expression.

b.

Summary Introduction

To explain:

The Fire and Mello experiment in which they injected double-stranded RNA complementary to the introns and promoter sequences of the gfp gene.

Introduction:

Gene expression is provides the information from DNA to RNA to protein, it is process in which the information of gene is used in the synthesis of a functional gene product. It involve the two stage, transcription, where the production of mRNA is done by using RNA polymerase and the processing of the resulting mRNA molecule. The gfp is a green fluorescent protein, it used as reporter gene in the experiment to determine the gene expression.

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