The use of probes in screening DNA libraries. Introduction: DNA library is collection of DNA fragments that cloned in a vector, there are two kinds of libraries genomic DNA and cDNA libraries. Genomic library is a collection of total genomic DNA from single organism, the DNA is stored in the vector, each vector contained different inserted DNA. The cDNA contain all cloned cDNA fragments are inserted into the host cell, which combined and makes some portion of the transcriptome of the organism.

Question

Want to see the full answer?

Answer 1

Question

Chapter 19.4, Problem 13COQ

Summary Introduction

To explain: The use of probes in screening DNA libraries.

Introduction:

DNA library is collection of DNA fragments that cloned in a vector, there are two kinds of libraries genomic DNA and cDNA libraries. Genomic library is a collection of total genomic DNA from single organism, the DNA is stored in the vector, each vector contained different inserted DNA. The cDNA contain all cloned cDNA fragments are inserted into the host cell, which combined and makes some portion of the transcriptome of the organism.

Summary Introduction

To explain: The preparation of synthetic probe when the protein product of the gene is known.

Introduction:

Synthetic probe is fragment of DNA; RNA of variable length approx 100−1000bp used to screen a DNA library, probes can be labeled with radioactive compound to detect the location of the probe.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

Green Algae, as a group, is actually paraphyletic with one subgroup more closely related to higher plants than the other. Which of the following green algae groups is more closely related to higher plants: a. Charophyceans b. Chlorophyceans c. Rhodophyta d. Xanthophyceans

A single-celled green algal genus that is motile with 2 flagella, has a cup shaped chloroplast, and an eyespot: a. Volvox b. Chlamydomonas c. Euglena d. Codium

A[n] ___ is produced by members of the Myxomycota when there is a lack of moisture. a. plasmodiocarp b. aethalium c. sclerotium d. plasmodium

Answer 2

Question

Chapter 19.4, Problem 13COQ

Summary Introduction

To explain: The use of probes in screening DNA libraries.

Introduction:

DNA library is collection of DNA fragments that cloned in a vector, there are two kinds of libraries genomic DNA and cDNA libraries. Genomic library is a collection of total genomic DNA from single organism, the DNA is stored in the vector, each vector contained different inserted DNA. The cDNA contain all cloned cDNA fragments are inserted into the host cell, which combined and makes some portion of the transcriptome of the organism.

Summary Introduction

To explain: The preparation of synthetic probe when the protein product of the gene is known.

Introduction:

Synthetic probe is fragment of DNA; RNA of variable length approx 100−1000bp used to screen a DNA library, probes can be labeled with radioactive compound to detect the location of the probe.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 3

Question

Chapter 19.4, Problem 13COQ

Summary Introduction

To explain: The use of probes in screening DNA libraries.

Introduction:

DNA library is collection of DNA fragments that cloned in a vector, there are two kinds of libraries genomic DNA and cDNA libraries. Genomic library is a collection of total genomic DNA from single organism, the DNA is stored in the vector, each vector contained different inserted DNA. The cDNA contain all cloned cDNA fragments are inserted into the host cell, which combined and makes some portion of the transcriptome of the organism.

Summary Introduction

To explain: The preparation of synthetic probe when the protein product of the gene is known.

Introduction:

Synthetic probe is fragment of DNA; RNA of variable length approx 100−1000bp used to screen a DNA library, probes can be labeled with radioactive compound to detect the location of the probe.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 19.4, Problem 13COQ

Summary Introduction

To explain: The use of probes in screening DNA libraries.

Introduction:

DNA library is collection of DNA fragments that cloned in a vector, there are two kinds of libraries genomic DNA and cDNA libraries. Genomic library is a collection of total genomic DNA from single organism, the DNA is stored in the vector, each vector contained different inserted DNA. The cDNA contain all cloned cDNA fragments are inserted into the host cell, which combined and makes some portion of the transcriptome of the organism.

Summary Introduction

To explain: The preparation of synthetic probe when the protein product of the gene is known.

Introduction:

Synthetic probe is fragment of DNA; RNA of variable length approx 100−1000bp used to screen a DNA library, probes can be labeled with radioactive compound to detect the location of the probe.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 5

Chapter 19.4, Problem 13COQ

Summary Introduction

To explain: The use of probes in screening DNA libraries.

Introduction:

DNA library is collection of DNA fragments that cloned in a vector, there are two kinds of libraries genomic DNA and cDNA libraries. Genomic library is a collection of total genomic DNA from single organism, the DNA is stored in the vector, each vector contained different inserted DNA. The cDNA contain all cloned cDNA fragments are inserted into the host cell, which combined and makes some portion of the transcriptome of the organism.

Summary Introduction

To explain: The preparation of synthetic probe when the protein product of the gene is known.

Introduction:

Synthetic probe is fragment of DNA; RNA of variable length approx 100−1000bp used to screen a DNA library, probes can be labeled with radioactive compound to detect the location of the probe.

Answer 6

Chapter 19.4, Problem 13COQ

Summary Introduction

To explain: The use of probes in screening DNA libraries.

Introduction:

DNA library is collection of DNA fragments that cloned in a vector, there are two kinds of libraries genomic DNA and cDNA libraries. Genomic library is a collection of total genomic DNA from single organism, the DNA is stored in the vector, each vector contained different inserted DNA. The cDNA contain all cloned cDNA fragments are inserted into the host cell, which combined and makes some portion of the transcriptome of the organism.

Answer 7

Chapter 19.4, Problem 13COQ

Summary Introduction

To explain: The use of probes in screening DNA libraries.

Introduction:

DNA library is collection of DNA fragments that cloned in a vector, there are two kinds of libraries genomic DNA and cDNA libraries. Genomic library is a collection of total genomic DNA from single organism, the DNA is stored in the vector, each vector contained different inserted DNA. The cDNA contain all cloned cDNA fragments are inserted into the host cell, which combined and makes some portion of the transcriptome of the organism.

Answer 8

Summary Introduction

To explain: The preparation of synthetic probe when the protein product of the gene is known.

Introduction:

Synthetic probe is fragment of DNA; RNA of variable length approx 100−1000bp used to screen a DNA library, probes can be labeled with radioactive compound to detect the location of the probe.

Answer 9

Summary Introduction

To explain: The preparation of synthetic probe when the protein product of the gene is known.

Introduction:

Synthetic probe is fragment of DNA; RNA of variable length approx 100−1000bp used to screen a DNA library, probes can be labeled with radioactive compound to detect the location of the probe.

Answer 10

Expert Solution & Answer

Answer 11

Expert Solution & Answer

Answer 12

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 13

Ch. 19.1 - Prob. 1COQ Ch. 19.1 - Prob. 1TPSQ Ch. 19.2 - Prob. 1CC Ch. 19.2 - Prob. 2CC Ch. 19.2 - Prob. 3CC Ch. 19.2 - Prob. 2COQ Ch. 19.2 - Prob. 3COQ Ch. 19.2 - Prob. 4COQ Ch. 19.2 - Prob. 5COQ Ch. 19.2 - Prob. 6COQ

Want to see the full answer?

Chapter 19 Solutions