Chapter 19.3, Problem 5TPSQ

Summary Introduction

To determine:

Advantages and disadvantages of gene cloning and PCR

Introduction:

PCR is used to make copies or amplification of DNA. It is of many types like, real time PCR, nested PCR, and semi- nested PCR. PCR is performed in three main steps, denaturing, annealing, and extension. PCR reaction contains a template DNA, primers, deoxynucleotide triphosphates, DNA polymerase, Mg ${}^{2+}$ ions, and buffer.

Gene cloning is a molecular technique in which one can make copies of DNA by inserting DNA into a vector like plasmid. It is done by the help of restriction enzymes, DNA ligases and vector.

Both these methods are used to make copies of DNA. DNA amplification is the process by which gene cloning or PCR make copies of DNA.