Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 19.6, Problem 2MQ
- What surprising finding has come out of many molecular studies of natural habitats using 16S rRNA as the target gene?
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
What surprising finding has come out of many molecular studiesof natural habitats using 16S rRNA as the target gene?
Carl Woese discovered that genes encoded for rRNAs are good candidates for phylogenetic analysis. Determine at least four characteristics/properties of these rRNA genes that make them effective molecular chronometers.
A scientist used a transgenic strain of C. elegans into
which a gene (gfp) for a green fluorescent protein had
been introduced. He injected some worms with
double-stranded RNA complementary to coding
sequence of the gfp gene. The figure below shows the
worms without (a; left) and with (b; right) ds gfp RNA.
a
(-) ds gfp RNA
_(+) ds gfp RNA
a) Explain these results .
b) The scientist conducted another experiment in which
he injected double-stranded RNA complementary to
the introns and promoter sequences of the gfp gene.
What results would you expect with this experiment?
Explain your answer .
Chapter 19 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 19.1 - Describe the enrichment strategy behind...Ch. 19.1 - Why is sulfate (So42) added to a Winogradsky...Ch. 19.1 - What is enrichment bias? How does dilution reduce...Ch. 19.1 - Why do the results of a direct enrichment of an...Ch. 19.2 - What is a pure culture and why is obtaining one...Ch. 19.2 - How does the agar dilution method differ from...Ch. 19.2 - What criteria serve to demonstrate that a culture...Ch. 19.3 - How might you isolate a morphologically unique...Ch. 19.3 - What is meant by high-throughput in culturing...Ch. 19.3 - What feature of high-throughput culturing relieves...
Ch. 19.4 - How does viability staining differ from stains...Ch. 19.4 - What types of environments limit the application...Ch. 19.4 - Why is it incorrect to say that the GFP is a...Ch. 19.4 - Prob. 1CRCh. 19.5 - What structure in the cell is the target for...Ch. 19.5 - FISH and CARD-FISH can be used to reveal different...Ch. 19.5 - Why is CARD-FISH more suitable than FISH for...Ch. 19.6 - What could you conclude from PCR/DGGE analysis of...Ch. 19.6 - What surprising finding has come out of many...Ch. 19.6 - How has next-generation sequencing technology...Ch. 19.6 - QWhich method, ARISA or T-RFLP, would provide more...Ch. 19.7 - Prob. 1MQCh. 19.7 - What are the advantages and disadvantages of...Ch. 19.7 - Why might a microarray be superior to using...Ch. 19.8 - Prob. 1MQCh. 19.8 - How do environmental genomic approaches differ...Ch. 19.8 - Prob. 3MQCh. 19.8 - Prob. 1CRCh. 19.9 - Prob. 1MQCh. 19.9 - If a large pulse of organic matter entered the...Ch. 19.9 - Q What are the major advantages of radioisotopic...Ch. 19.10 - What is the simplest explanation for why lunar...Ch. 19.10 - What is the expected isotopic composition of...Ch. 19.10 - How might exchange of metabolites among members of...Ch. 19.10 - Will autotrophic organisms contain more or less...Ch. 19.11 - How could NanoSIMS be used to identify a...Ch. 19.11 - Prob. 2MQCh. 19.11 - How does MAR-FISH link microbial diversity and...Ch. 19.11 - Q What can MAR-FISH tell you that FISH alone...Ch. 19.12 - How can stable isotope probing reveal the identity...Ch. 19.12 - What key method is required to do genomics on a...Ch. 19.12 - Prob. 3MQCh. 19.12 - How would you use cytometric cell sorting to...Ch. 19 - Design an experiment for measuring the activity of...Ch. 19 - You wish to know whether Archaea exist in a lake...Ch. 19 - Design an experiment to solve the following...Ch. 19 - Design a SIP experiment that would allow you to...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- a)What is Figure 1 in the Zheng et al paper showing? Describe it in your own words. b) What are some sections of the Zheng paper that are re-arranged or different than you might expect in a primary research article. Is this still primary research? c) How did the authors use CRISPR to alter the butterfly wing expression? What was the result of this use of CRISPR?arrow_forwardHow is ribotyping similar to rRNA sequence analysis? How do the two techniques differ? Do you think one is more accurate than the other?arrow_forwardThe phylogenetic tree of the OCTN homologs below was generated with the following accession numbers as basis: NP_957143.1 - OCTN of unknown function CDM63451.1 - OCTN specific for ergothioneine transport XP_021324939.1 - OCTN specific for carnitine transport Design the phylogenetic tree to make it look more interesting by highlighting the distinct clades or putting a visual emphasis on the gene you are trying to analyze. Provide a caption for this figure.arrow_forward
- The phylogenetic tree of the OCTN homologs below was generated with the following accession numbers as basis: NP_957143.1 - OCTN of unknown function CDM63451.1 - OCTN specific for ergothioneine transport XP_021324939.1 - OCTN specific for carnitine transport Design the phylogenetic tree to make it look more interesting by highlighting the distinct clades or putting a visual emphasis on the gene you are trying to analyze. Provide a caption for this figure. asap please.arrow_forwardIn metagenomic studies, a comparison of ribosomal RNA sequences is often used to determine the number of different species present. What are some characteristics of ribosomal sequences that make them useful for determining what species are present?arrow_forward(a) What is Figure 1 in the Zheng et al paper showing? Describe it in your own words. (b) What are some sections of the Zheng paper that are re-arranged or different than you might expect in a primary research article. Is this still primary research? (c) How did the author use CRISPR to alter the butterfly wing expression? What was the result of this use of CRISPR?arrow_forward
- Bacteriophage lambda is as one of the routinely used molecular cloning vectors, which alsoserved as a model system for the study of bacteriophage morphogenesis, DNA replication, andgene regulation.a) With the aid of a diagram, generally narrate how a foreign gene can be inserted into alambda insertion vector and subsequently infect an Escherichia coli cell.b) You are cloning a 7.5 kb gene into a lambda gt10 vector, utilizing a restriction site whichspecifically present in the vector. State the restriction site that you can use for this purposeand suggest a screening procedure to indicate successful integration of the gene into thehost genome.arrow_forwardConserved gene order can be termed asa) Orthologb) Syntenyc) Paralogd) Microarrayarrow_forwardwhat is the purpose of this experiment and the hypothesis of the paper?arrow_forward
- With respect to wobble hypothesis all of the following are correct except: An inosine nucleotide in the mRNA molecule can base-pair with A, C, and U in the tRNA The anticodon shows both standard and non-standard base pairing with the codon at wobble position Guanine can base-pair with uracil An inosine nucleotide in the tRNA molecule can base-pair with A, C and U in the mRNAarrow_forwardWhat is the significance of digesting the 16S rRNA product in 16s gene sequencing?arrow_forwardSuppose a researcher previously cloned gene Y into M13 bacteriophage vector. Gene Y encodes a product called peptide Y. A region of gene Y contains the DNA sequence ATG-CGC-GAA-CTG-GTG-AAC-TAA. The researcher wishes to change a Val residue to an Ala residue in this region of peptide Y using site-directed mutagenesis. What should be the sequence of the mutant oligonucleotide primer in this region? You may use a codon table. mutant oligonucleotide primer sequence: GGC-GGC-GAA-CTG-GTG-AAC-TAA Incorrectarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
QCE Biology: Introduction to Gene Expression; Author: Atomi;https://www.youtube.com/watch?v=a7hydUtCIJk;License: Standard YouTube License, CC-BY