Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Textbook Question
Chapter 19.1, Problem 1CR
Why do the results of a direct enrichment of an environmental sample and enrichment following dilution of the sample often differ with respect to the types of populations recovered?
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Chapter 19 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 19.1 - Describe the enrichment strategy behind...Ch. 19.1 - Why is sulfate (So42) added to a Winogradsky...Ch. 19.1 - What is enrichment bias? How does dilution reduce...Ch. 19.1 - Why do the results of a direct enrichment of an...Ch. 19.2 - What is a pure culture and why is obtaining one...Ch. 19.2 - How does the agar dilution method differ from...Ch. 19.2 - What criteria serve to demonstrate that a culture...Ch. 19.3 - How might you isolate a morphologically unique...Ch. 19.3 - What is meant by high-throughput in culturing...Ch. 19.3 - What feature of high-throughput culturing relieves...
Ch. 19.4 - How does viability staining differ from stains...Ch. 19.4 - What types of environments limit the application...Ch. 19.4 - Why is it incorrect to say that the GFP is a...Ch. 19.4 - Prob. 1CRCh. 19.5 - What structure in the cell is the target for...Ch. 19.5 - FISH and CARD-FISH can be used to reveal different...Ch. 19.5 - Why is CARD-FISH more suitable than FISH for...Ch. 19.6 - What could you conclude from PCR/DGGE analysis of...Ch. 19.6 - What surprising finding has come out of many...Ch. 19.6 - How has next-generation sequencing technology...Ch. 19.6 - QWhich method, ARISA or T-RFLP, would provide more...Ch. 19.7 - Prob. 1MQCh. 19.7 - What are the advantages and disadvantages of...Ch. 19.7 - Why might a microarray be superior to using...Ch. 19.8 - Prob. 1MQCh. 19.8 - How do environmental genomic approaches differ...Ch. 19.8 - Prob. 3MQCh. 19.8 - Prob. 1CRCh. 19.9 - Prob. 1MQCh. 19.9 - If a large pulse of organic matter entered the...Ch. 19.9 - Q What are the major advantages of radioisotopic...Ch. 19.10 - What is the simplest explanation for why lunar...Ch. 19.10 - What is the expected isotopic composition of...Ch. 19.10 - How might exchange of metabolites among members of...Ch. 19.10 - Will autotrophic organisms contain more or less...Ch. 19.11 - How could NanoSIMS be used to identify a...Ch. 19.11 - Prob. 2MQCh. 19.11 - How does MAR-FISH link microbial diversity and...Ch. 19.11 - Q What can MAR-FISH tell you that FISH alone...Ch. 19.12 - How can stable isotope probing reveal the identity...Ch. 19.12 - What key method is required to do genomics on a...Ch. 19.12 - Prob. 3MQCh. 19.12 - How would you use cytometric cell sorting to...Ch. 19 - Design an experiment for measuring the activity of...Ch. 19 - You wish to know whether Archaea exist in a lake...Ch. 19 - Design an experiment to solve the following...Ch. 19 - Design a SIP experiment that would allow you to...
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- Distinguish between pre-enrichment, enrichment and selective enrichment liquid cultures.arrow_forwardExplain the Biozhazards that may occur, when performer performing analysis of biological samples on Mass Spectromtery. Note: Please provide typed solution, not handwritten. Thumbs up in advance...arrow_forwardWhat are advantages and disadvantages of solid phase bioremediation technique?arrow_forward
- There are different types of sampling designs that may be used depending on the types of studies. Which sampling design you may recommend for the following experiments : a. To investigate the disease incidence in a field of tomato b. To investigate the nosocomial infection in people c. To investigate the microbial diversity in an agricultural fieldarrow_forwardWhat is the purpose of the blank test in the experiments?arrow_forwardAre you able to identify the lag, log, stationary and death phase in using these growth curves?Are you able to calculate the generation time for this microbe under the two conditions?arrow_forward
- What is an advantage of in-situ bioremediation as a means of eliminating contaminants over chemical decomposition and excavation?arrow_forward3. (a) Comparing the culture-dependant (e.g., colony forming units count) and DNA- based (e.g., PCR) bacterial number quantification methods, what are the primary disadvantages of using culture-dependent methods to quantify bacterial numbers in an environmental sample? Use no more than three sentences or bullet points to present your answer succinctly.arrow_forwardExplain each four steps in Product Development that was given below: a.) Isolation of microbes that produce product of interest. b.) Screening for best producing strain;naturally or mutation or genetic engineering c.)Optimization of production condition d.)Scaling up from lab scale ( up to 10L) to industrial scale (> 10,000L)arrow_forward
- How can the enrichment culture technique be used medically?arrow_forwardWhat are the expected problems in the centrifuge in case of imbalance of samples ? *arrow_forwardWhich of the following types of data produce curves similar in shape to the graph? Select all that apply 1.Bacterial growth in a culture 2.A normal distribution of height in a population of students 3.Exponential population growth 4.Haemoglobin binding of oxygen 5.Log transformations of drug dose curves 6.Osmotic fragility of red blood cells 7.A standard curve for an enyme assayarrow_forward
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