Chapter 19.4, Problem 1CR

Summary Introduction

In staining method, various dyes (acridine orange, DAPI, and SYBR Green) are used to strain microorganisms and that can be visualized under microscope. Genetic engineering technology is used to alter bacterial cells to produce autofluorescence by inserting the gene encoding green fluorescent protein (GFP) into the bacterial genome. Bacterial cells fluoresce green if the GFP gene is expressed and the fluorescence can be visualized under ultraviolet microscopy.