Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Textbook Question
Chapter 19.5, Problem 1CR
Why is CARD-FISH more suitable than FISH for characterizing very slowly growing microorganisms in the environment?
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
Why is it important for all plankton monitors to use the sample protocol for both sample collection and identification?
The process of collecting characteristics of your unknown bacterium can be helped by the use of a dichotomous key. A dichotomous key is a flowchart that can be used to identify an organism.
a) in your own words explain how to create and use a dichotomous key to identify unknown bacteria
propose a hypothesis regarding the organisms (staphylococcus, escherichia, pseudomonas, and bacillus) that will be resistant to the most disinfectants, and why?
if the hypothesis is supported, what specific experimental results will be observed?
what would be the independent and dependent variable? zone size, disinfectant type, colony count
Chapter 19 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 19.1 - Describe the enrichment strategy behind...Ch. 19.1 - Why is sulfate (So42) added to a Winogradsky...Ch. 19.1 - What is enrichment bias? How does dilution reduce...Ch. 19.1 - Why do the results of a direct enrichment of an...Ch. 19.2 - What is a pure culture and why is obtaining one...Ch. 19.2 - How does the agar dilution method differ from...Ch. 19.2 - What criteria serve to demonstrate that a culture...Ch. 19.3 - How might you isolate a morphologically unique...Ch. 19.3 - What is meant by high-throughput in culturing...Ch. 19.3 - What feature of high-throughput culturing relieves...
Ch. 19.4 - How does viability staining differ from stains...Ch. 19.4 - What types of environments limit the application...Ch. 19.4 - Why is it incorrect to say that the GFP is a...Ch. 19.4 - Prob. 1CRCh. 19.5 - What structure in the cell is the target for...Ch. 19.5 - FISH and CARD-FISH can be used to reveal different...Ch. 19.5 - Why is CARD-FISH more suitable than FISH for...Ch. 19.6 - What could you conclude from PCR/DGGE analysis of...Ch. 19.6 - What surprising finding has come out of many...Ch. 19.6 - How has next-generation sequencing technology...Ch. 19.6 - QWhich method, ARISA or T-RFLP, would provide more...Ch. 19.7 - Prob. 1MQCh. 19.7 - What are the advantages and disadvantages of...Ch. 19.7 - Why might a microarray be superior to using...Ch. 19.8 - Prob. 1MQCh. 19.8 - How do environmental genomic approaches differ...Ch. 19.8 - Prob. 3MQCh. 19.8 - Prob. 1CRCh. 19.9 - Prob. 1MQCh. 19.9 - If a large pulse of organic matter entered the...Ch. 19.9 - Q What are the major advantages of radioisotopic...Ch. 19.10 - What is the simplest explanation for why lunar...Ch. 19.10 - What is the expected isotopic composition of...Ch. 19.10 - How might exchange of metabolites among members of...Ch. 19.10 - Will autotrophic organisms contain more or less...Ch. 19.11 - How could NanoSIMS be used to identify a...Ch. 19.11 - Prob. 2MQCh. 19.11 - How does MAR-FISH link microbial diversity and...Ch. 19.11 - Q What can MAR-FISH tell you that FISH alone...Ch. 19.12 - How can stable isotope probing reveal the identity...Ch. 19.12 - What key method is required to do genomics on a...Ch. 19.12 - Prob. 3MQCh. 19.12 - How would you use cytometric cell sorting to...Ch. 19 - Design an experiment for measuring the activity of...Ch. 19 - You wish to know whether Archaea exist in a lake...Ch. 19 - Design an experiment to solve the following...Ch. 19 - Design a SIP experiment that would allow you to...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Is it acceptable to give a formal name to a microbe that hasn't been isolated and cultivated? What type of name should a microorganism have if it has been well identified but cannot yet be cultured in isolation?arrow_forwardWhy are macroscopic methods such as motility agar and colonial morphologic examination useful for classifying bacteria?arrow_forwardWhich of the following is NOT correctly matched? A. Halophile – microbe that can grow in an environment with high osmotic pressure (e.g., salt) B. None of the other four answers (all are correctly matched) C. Agar – complex polysaccharide from seaweed used as a solidifying agent in culture media D. Colony – a population of microbial cells arising from a single cell or spore or from a group of attached cells E. Capnophile – microbe requiring an elevated molecular oxygen (O2) environment for optimal growtharrow_forward
- Lead is a very toxic metal that is not easily degraded in the environment. Lead pipes, paint containing lead, car batteries, and even lead fishing sinkers are all potential sources for the release of lead into the environment. what possible strategy can be done for identifying bacteria that may play a role in degrading lead?arrow_forwardWhat is G+C content of a bacteria? How it can be determined? Why it is not safe to assume that microorganisms with same G+C content belong to same species? How G+C content data is taxonomically valuable?arrow_forwardBacterial growth depends on many environmental factors, including the temperature of the environment. Since microbes can survive in a certain range of temperatures and will thrive at a temperature, understanding these variables allows for control of their growth. This understanding can be used, for example, to preserve certain foods or to treat infections. This lab simulation will use an instrument called a spectrophotometer. This instrument quantitatively measures the amount of light that is absorbed or transmitted by molecules in solution. Microbial populations grow at different rates based on temperature. One way to measure growth is by turbidity measurements. When bacteria multiply in a broth culture they turn the clear solution cloudy, or turbid. a) in your own words explain the proper steps to Inoculate 5°C sterile broth tube using aseptic techniquearrow_forward
- Attached below is the study about the effects of handwashing and the effectiveness of soap against bacteria. What conclusions can be generated from this result? A C before washing hands 10 second hand wash B D hot water only rinse 30 second hand wash Figure 8: Agar plates with bacteria A) before washing hands B) after rinsing hands in hot water C) After washing hands with soap for 10 seconds and D) after washing hands for 30 seconds. Images show that the number of bacteria on the plate are not greatly reduced after a hot water rinse or 10 second handwash but bacterial levels are reduced after a proper hand wash for 30 seconds.arrow_forwardWhy might bacteria use quorum sensing to regulate genes needed for virulence? How might this reason be related to the rationale behind using quorum sensing to establish a symbiotic relationship?arrow_forwardSuppose you do this test on a hypothetical Staphylococcal species with the antibiotics penicillin and tetracycline. You record zone diameters of 20mm for the tetracycline disc and 25mm for the penicillin disc. Which antibiotic would be most effective against this organism? What does this tell you about comparing zone diameters to each other and the importance of the interpretive chart?arrow_forward
- since gram-negative bacteria are generally more resistant to antibiotics such as penicillin, Gram staining can be used to inform appropriate antibiotic treatment for patients with bacterial infections. Based on the information in the key (Dichotomous key), infections caused by bacteria belonging to which genera would be the most effectively treated with penicillin?arrow_forwardThe Gram staining technique is useful to classify Bacteria. Can the technique be applied to Archaea? Discuss briefly.arrow_forwardWhich among A-D is false regarding growth and/or analysis of a bacterial culture? A) O Fed-batch growth: typically results in an increased yield of cells over that obtained with batch growth B) O Auxotroph: requires addition of specific growth factors in order to grow C) O Prototroph: typically the wild type strain that possesses all the features indicative of the species D) O None of A-D is false; all are true statementsarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
cell culture and growth media for Microbiology; Author: Scientist Cindy;https://www.youtube.com/watch?v=EjnQ3peWRek;License: Standard YouTube License, CC-BY