The consequences of radio labeling with 1 4 CO 2 in the reductive TCA cycle should be determined. Concept Introduction: In the forward cycle, CO 2 will be lost whereas in reverse TCA cycle it will be incorporated which will happen at the conversion of succinyl−CoA to α -ketoglutarate and again at the conversion of isocitrate to citrate.

Question

Pyruvate is accepted into the TCA cycle by a “feeder” reaction using the pyruvatedehydrogenase complex, resulting in acetyl-CoA and CO2. Provide a full mechanismfor this reaction utilizing the TPP cofactor. Include the roles of all cofactors.

Answer 1

Question

Chapter 19, Problem 12P

Interpretation Introduction

Interpretation:

The consequences of radio labeling with ¹⁴CO₂ in the reductive TCA cycle should be determined.

Concept Introduction:

In the forward cycle, CO₂ will be lost whereas in reverse TCA cycle it will be incorporated which will happen at the conversion of succinyl−CoA to α -ketoglutarate and again at the conversion of isocitrate to citrate.

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