The ratio of [isocitrate]/[citrate] in given mitochondria and the [citrate] should be calculated. Concept Introduction: The free energy, ΔG can be determined from the standard free energy change, Δ G 0 with the below reaction: Δ G= Δ G 0 + RT × ln [ isocitrate ] [ citrate ] Where R is universal gas constant, T is temperature and square brackets represent concentration.

Question

Pyruvate is accepted into the TCA cycle by a “feeder” reaction using the pyruvatedehydrogenase complex, resulting in acetyl-CoA and CO2. Provide a full mechanismfor this reaction utilizing the TPP cofactor. Include the roles of all cofactors.

Answer 1

Question

Chapter 19, Problem 10P

Interpretation Introduction

Interpretation:

The ratio of [isocitrate]/[citrate] in given mitochondria and the [citrate] should be calculated.

Concept Introduction:

The free energy, ΔG can be determined from the standard free energy change, ΔG0 with the below reaction:

ΔG=ΔG0+ RT×ln[isocitrate][citrate]

Where R is universal gas constant, T is temperature and square brackets represent concentration.

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