Biochemistry
Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 19, Problem 10P
Interpretation Introduction

Interpretation:

The ratio of [isocitrate]/[citrate] in given mitochondria and the [citrate] should be calculated.

Concept Introduction:

The free energy, ΔG can be determined from the standard free energy change, ΔG0 with the below reaction:

  ΔG=ΔG0+ RT×ln[isocitrate][citrate]

Where R is universal gas constant, T is temperature and square brackets represent concentration.

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M-CSA Mechanism and Catalytic Site Atlas (ebi.ac.uk) (ii) Acyl Carrier Protein S-acetyltransferase (EC 2.3.1.38) is a transferase enzyme that catalyzes the first biosynthetic pathway for fatty acid synthase. It transfers the acyl group (CH3CO) first from coenzyme A to a cysteine residue in the active site. This is similar to what happens in Chymotrypsin, however utilizing a sulfur instead of an oxygen. The acyl group is then transferred to the molecule ACP. Provide the enzyme- catalyzed mechanism for the reaction below, making sure to identify the roles of all key amino acids: i H3C SCOA acetyl COA enzyme + HS i H3C SACP acetyl ACP
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