EBK GENETICS: FROM GENES TO GENOMES
6th Edition
ISBN: 9781260041255
Author: HARTWELL
Publisher: MCGRAW HILL BOOK COMPANY
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 18, Problem 20P
One way to determine where inside a cell a protein (protein X) normally localizes is to generate a reporter gene construct containing: (i) the gene X regulatory region and coding sequences, and (ii) coding sequences for GFP fused in frame to the 3′ end of the gene X coding sequences just before the stop codon. A mouse containing such a transgene will express a hybrid protein X-GFP only in those cells in which gene X is normally expressed.
| a. | The gene X-GFP fusion gene described could be generated by knocking in GFP coding sequences instead of by random insertion of a transgene. Diagram the knockin construct you could use for this purpose. |
| b. | What might the advantage be of the knockin strategy versus the transgene strategy? |
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Luke recently moved to a new apartment and wants to grow houseplants but isn't sure which room will be the best fit for them. Apply your knowledge of the scientific method to recommend a strategy for Luke to follow when determining the ideal location for houseplants in his new apartment.
A farmer has noticed that his soybean plants produce more beans in some years than others. He claims to always apply the same amount of fertilizer to the plants, but he suspects the difference in crop yield may have something to do with the amount of water the crops receive. The farmer has observed that the soybeans on his farm usually receive between 0 to 0.5 inches of water per day, but he is unsure of the optimal average daily amount of water with which to irrigate.
1. State a question that addresses the farmer’s problem
2. Conduct online research on “soybean crop irrigation" and record a brief summary of the findings
3. Construct a testable hypothesis and record i
4. Design an experiment to test the hypothesis and describe the procedures, variables, and data to be collected
5. What is the purpose of a control group in an experiment? What would the control groups be for each of your designed experiments in this exercise?
6. Describe the data that would be recorded in each of the…
A farmer has noticed that his soybean plants produce more beans in some years than others. He claims to always apply the same amount of fertilizer to the plants, but he suspects the difference in crop yield may have something to do with the amount of water the crops receive. The farmer has observed that the soybeans on his farm usually receive between 0 to 0.5 inches of water per day, but he is unsure of the optimal average daily amount of water with which to irrigate.
1. State a question that addresses the farmer’s problem
2. Conduct online research on “soybean crop irrigation" and record a brief summary of the findings
3. Construct a testable hypothesis and record i
4. Design an experiment to test the hypothesis and describe the procedures, variables, and data to be collected
Chapter 18 Solutions
EBK GENETICS: FROM GENES TO GENOMES
Ch. 18 - Match each of the terms in the left column to the...Ch. 18 - Mice are usually gray, but a mouse geneticist has...Ch. 18 - Sometimes, genes transferred into the mouse genome...Ch. 18 - In mice, a group of so-called Hox genes encode...Ch. 18 - The fly eyes shown in Fig. 18.7 are malformed...Ch. 18 - This problem concerns a technique called enhancer...Ch. 18 - Fish and other organisms that live in the Arctic...Ch. 18 - a. Describe two ways you could potentially make a...Ch. 18 - Figure 18.6 shows a picture of Glofish ,...Ch. 18 - Some people are concerned about the possible...
Ch. 18 - The goal of the Knockout Mouse Project is to...Ch. 18 - Prob. 12PCh. 18 - Prob. 13PCh. 18 - a. Which genome manipulation technique would you...Ch. 18 - a. Diagram a knockin construct that could have...Ch. 18 - Prob. 16PCh. 18 - Prob. 17PCh. 18 - The transcription factor Pax6 is required...Ch. 18 - Mouse models for human genetic diseases are...Ch. 18 - One way to determine where inside a cell a protein...Ch. 18 - In Problem 5 in Chapter 17, you saw that a SNP...Ch. 18 - Scientists now routinely use CRISPR/Cas9 to make...Ch. 18 - Geneticists are currently considering using...Ch. 18 - a. Figures 18.9 and 18.12 demonstrated methods to...Ch. 18 - Nonhomologous end-joining NHEJ of a double-strand...Ch. 18 - One problem that researchers sometimes encounter...Ch. 18 - Researchers at the University of California at San...Ch. 18 - Prob. 28PCh. 18 - F. Port and S. Bullock at the University of...Ch. 18 - On Fig 18.14, locate the PAM site and identify the...Ch. 18 - Prob. 31PCh. 18 - Prob. 32PCh. 18 - Recall that Leber congenital amaurosis LCA, a form...Ch. 18 - One potential strategy for gene therapy to correct...Ch. 18 - Recently, scientists have used a mouse model for...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- A pharmaceutical company has developed a new weight loss drug for adults. Preliminary tests show that the drug seems to be fairly effective in about 75% of test subjects. The drug company thinks that the drug might be most effective in overweight individuals, but they are unsure to whom they should market the product. Use the scientific method to address the pharmaceutical company’s needs: State a research question that addresses the pharmaceutical company's problem Conduct online research on “Body Mass Index” categories and record a brief summary Construct a testable hypothesis and record in Design an experiment to test the hypothesis and describe the procedures, variables, and data to be collected What is the purpose of a control group in an experiment? What would the control groups be for each of your designed experiments in this exercise? Describe the data that would be recorded in each of the experiments you designed. Would it be classified as quantitative or…arrow_forwardPatients with multiple sclerosis frequently suffer from blurred vision. Drug X was developed to reduce blurred vision in healthy patients, but the effectiveness had not been tested on those suffering from multiple sclerosis. A study was conducted to determine if Drug X is effective at reducing blurry vision in multiple sclerosis patients. To be considered effective, a drug must reduce blurred vision by more than 30% in patients. Researchers predicted that a 20 mg dose of the drug would be effective for treating blurred vision in multiple sclerosis patients by reducing blurred vision by more than 30%. Drug X was administered to groups of multiple sclerosis patients at three doses (10 mg/day, 20 mg/day, 30 mg/day) for three weeks. A fourth group of patients was given a placebo containing no drug X for the same length of time. Vision clarity was measured for each patient before and after the three-week period using a standard vision test. The results were analyzed and graphed (See Figure…arrow_forwardSvp je voulais demander l aide pour mon exercicearrow_forward
- Imagine that you are a clinical geneticist. Your colleague is an oncologist who wants your help explaining the basics of genetics to their patient, who will be undergoing genetic testing in the coming weeks for possible acute myeloid leukemia (AML) induced by the radiation she had several years ago for breast cancer. Write a 1,050- to 1,225-word memo to your colleague. Include the following in your memo: An explanation of the molecular structure of DNA and RNA, highlighting both similarities and differences A description of the processes of transcription and translation An explanation of the differences between leading and lagging strands and how the DNA is replicated in each strand Reponses to the following common questions patients might ask about this type of genetic testing and genetic disorder: Does AML run in families? What genes are tested for?arrow_forwardRespond to the following in a minimum of 175 words: What are some potential consequences that could result if the processes of replication, transcription, and translation don’t function correctly? Provide an example of how you might explain these consequences in terms that patients might understand.arrow_forwardanswer questions 1-10arrow_forward
- Answer Question 1-9arrow_forwardEx: Mr. Mandarich wanted to see if the color of light shined on a planthad an effect on the number of leaves it had. He gathered a group ofthe same species of plants, gave them the same amount of water, anddid the test for the same amount of time. Only the color of light waschanged. IV:DV:Constants:Control Gr:arrow_forwardethical considerations in medical imagingarrow_forward
- Please correct answer and don't used hand raiting and don't used Ai solutionarrow_forward2. In one of the reactions of the citric acid cycle, malate is oxidized to oxaloacetate. When this reaction is considered in isolation, a small amount of malate remains and is not oxidized. The best term to explain this is a. enthalpy b. entropy c. equilibrium d. free energy e. loss of energyarrow_forward18. The citric acid cycle takes place in a. the chloroplasts b. the cytosol c. the inner mitochondrial membrane d. between the two mitochondrial membranes e. the mitochondrial matrix 40 WILarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage Learning
Biology (MindTap Course List)BiologyISBN:9781337392938Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. BergPublisher:Cengage Learning
Biology Today and Tomorrow without Physiology (Mi...BiologyISBN:9781305117396Author:Cecie Starr, Christine Evers, Lisa StarrPublisher:Cengage Learning
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Biology: The Dynamic Science (MindTap Course List)BiologyISBN:9781305389892Author:Peter J. Russell, Paul E. Hertz, Beverly McMillanPublisher:Cengage Learning
Human Heredity: Principles and Issues (MindTap Co...
Biology
ISBN:9781305251052
Author:Michael Cummings
Publisher:Cengage Learning
Biology (MindTap Course List)
Biology
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Cengage Learning
Biology Today and Tomorrow without Physiology (Mi...
Biology
ISBN:9781305117396
Author:Cecie Starr, Christine Evers, Lisa Starr
Publisher:Cengage Learning
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Cengage Learning
Bacterial Genomics and Metagenomics; Author: Quadram Institute;https://www.youtube.com/watch?v=_6IdVTAFXoU;License: Standard youtube license