EBK GENETICS: FROM GENES TO GENOMES
EBK GENETICS: FROM GENES TO GENOMES
6th Edition
ISBN: 9781260041255
Author: HARTWELL
Publisher: MCGRAW HILL BOOK COMPANY
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Chapter 18, Problem 12P
Summary Introduction

a.

To determine:

The diagram of a gene construct that can generate a strain with a particular knockout of histone subunit.

Introduction:

The process of gene targeting can be performed in the yeast cell. The sequence of the genome of the yeast cell is available for the researchers.

Summary Introduction

b.

To determine:

The steps performed for the generation of mutant yeast.

Introduction:

The process of gene targeting can be easily used for creating yeast strain with a particular knockout mutation in any gene.

Summary Introduction

c.

To determine:

Whether targeting of gene can be performed in haploid or diploid strain.

Introduction:

The knockout mutation is present in every gene inside the genome of the organism. In the knockout technique, the nonfunctional genes are silenced by replacing them with the functional gene.

Summary Introduction

d.

To determine:

The conclusion and the explanation of the expected result for H2A gene.

Introduction:

H2A gene in the diploid strain is being knocked out in the diploid strain for the generation of the heterozygote.

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Chapter 18 Solutions

EBK GENETICS: FROM GENES TO GENOMES

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Mitochondrial mutations; Author: Useful Genetics;https://www.youtube.com/watch?v=GvgXe-3RJeU;License: CC-BY