The showing off six spots in the separation of mixture of seven amino acids by chromatography should be explained. Concept introduction: Ninhydrin forms colored imine product when reacted with an amino acid. The mixture of amino acids can be separated by chromatography on the basis of their difference in polarities. Chromatography is technique to separate mixture of compounds by using a stationary phase and a mobile phase, in which mobile phase carry the compounds one by one on the basis of their polarities.

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Chapter 17.5, Problem 10P

Interpretation Introduction

Interpretation:

The showing off six spots in the separation of mixture of seven amino acids by chromatography should be explained.

Concept introduction:

Ninhydrin forms colored imine product when reacted with an amino acid.

The mixture of amino acids can be separated by chromatography on the basis of their difference in polarities.

Chromatography is technique to separate mixture of compounds by using a stationary phase and a mobile phase, in which mobile phase carry the compounds one by one on the basis of their polarities.

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A mixture of seven amino acids (glycine, glutamate, leucine, lysine, alanine, isoleucine, and aspartate) is separated by chromatography. Explain why only six spots show up when the chromatographic plate is coated with ninhydrin and heated.

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