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Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Chapter 17, Problem 2TYK
Summary Introduction
Introduction:
Bacterial conjugation is a process by which one bacterium (donor) transfers genetic material to another bacterial cell (recipient) under the control of a plasmid that facilitates direct cell-to-cell contact and by the formation of a bridge-like connection between two cells.
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Which of the following statements about conjugation is false?
After a F- cell has conjugated with an F+ cell, both cells would be F+.
Both Hfr and F+ bacteria have the ability to produce a sex pilus.
In F+ × F- conjugation, none of the bacterial chromosome is transferred; only the F factor is.
A Hfr strain usually transfers the entire bacterial chromosome to the recipient cell.
F' × F- conjugation can produce a partially diploid bacterial cell.
Explain the importance of the following features in conjugating donor bacteria:
a. the origin of transfer
b. the conjugation pilus
c. homologous recombination
d. the relaxosome
e. relaxase
f. T strand DNA
g. pillin protein
The following recombinants are recovered when
conjugation occurs between an a*d*g+ donor and an
adg recipient.
at d+ g+ = 84%
a d g+ = 6%
at d g+ = 10%
a dt g+ = less than 1%
What is the map distance between the a and d genes?
10 map units
74 map units
less than 1 map unit
84 map units
6 map units
Chapter 17 Solutions
Biology: The Dynamic Science (MindTap Course List)
Ch. 17.1 - What are the properties of F+, F-, and Hfr cells...Ch. 17.1 - Prob. 2SBCh. 17.2 - Prob. 1SBCh. 17.2 - How does viral infection of an animal cell and a...Ch. 17.2 - Prob. 3SBCh. 17.3 - Prob. 1SBCh. 17 - Prob. 1TYKCh. 17 - Prob. 2TYKCh. 17 - Which of the following is not correct for...Ch. 17 - Prob. 4TYK
Ch. 17 - Prob. 5TYKCh. 17 - Prob. 6TYKCh. 17 - When a virus enters the lysogenic stage: a. the...Ch. 17 - An infectious material is isolated from a nerve...Ch. 17 - Prob. 9TYKCh. 17 - Prob. 10TYKCh. 17 - Prob. 11TYKCh. 17 - Discuss Concepts As a control for their...Ch. 17 - Prob. 13TYKCh. 17 - Prob. 14TYKCh. 17 - Prob. 15TYKCh. 17 - Prob. 1ITD
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- Order the following experimental steps to identify auxotrophic mutants in Saccharomyces Cerevisiae that cannot sythesize their own Leucine. an option can be selected more than once. step 1: Step 2: Step 3: Step 4: Step 5: Step 6:arrow_forwardA series of Hfr strains that have genotype m+ n+ o+ p+ q+ r+ are mixed with an F− strain that has genotype m− n− o− p− q− r−. Conjugation is interrupted at regular intervals and the order of the appearance of genes from the Hfr strain is determined in the recipient cells. The order of gene transfer for each Hfr strain is What is the order of genes on the circular bacterial chromosome? For each Hfr strain, give the location of the F factor in the chromosome and its polarity. Hfr5 m+ q+ p+ n+ r+ o+ Hfr4 n+ r+ o+ m+ q+ p+ Hfr1 o+ m+ q+ p+ n+ r+ Hfr9 q+ m+ o+ r+ n+ p+arrow_forwardYou have conducted a transposon mutagenesis experiment using the same PRL27 system that was used during your lab exercise. After allowing for conjugation. you plate the conjugation mix on Luria agar and Luria + Kanamycin agar. After incubation, you count the following number of colonies on each type of plate: Media Dilution Number of Colonies Too Many to Luria 10-5 Count 10-6 173 10-7 28 Luria + Kanamycin 100 324 10-1 217 10-2 3 Determine the transformation efficiency. Note: You can enter you answer using long form (e.g. 1500000) or scientific notation using e notation (e.g. 1.5e6). Do not enter units.arrow_forward
- First picture: 1. What must be added in a minimal media for the recombinants to grow? 2. What must be excluded in a minimal media for the non recombinants to be killed? Second picture: 1. How is the recipient cell different at time D than it was at time A? Choice 1:It has a greater number of genes Choice 2: It has a greater mass of DNA. Choice 3:It contains different genes Choice 4: It contains bacteriophage DNA. 2. At which time can the recipient cell first be described as "recombinant"? A,B,C, or D 3. Which of the following processes is responsible for the shape of the curve at time B? Choice 1:transduction Choice 2:another choice Choice 3:entry of Hfr DNA into the recipient cell Choice 4:entry of DNA from the recipient cell into the Hfr cellarrow_forwardA bacterial transformation is performed with a donor strain that is resistant to four drugs, A, B, C, and D, and a recipient strain that is sensitive to all four drugs. The resulting recipient cell population is divided and plated onmedia containing various combinations of the drugs. The following table shows the results.a. One of the genes is distant from the other three, which appear to be closely linked. Which is the distant gene? b. What is the likely order of the three closely linked genesarrow_forwardSelect the following statements that DO apply to the process of non-homologous end joining. It can be error prone. D A. O B. It uses a sister chromatid as a template to repair a damaged chromosome. It can sometimes result in base deletions. AD It is a process that is only observed in mouse cells. OE It is a way to assemble recombinant DNA plasmids in vitro. OE It is a mechanism by which cells correct double strand breaks in DNA.arrow_forward
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