Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Chapter 17, Problem 1ITD
Summary Introduction
To review:
The construction of the map to show the arrangement of genes in a bacterial genome.
Introduction:
Different strains of high-frequency recombinant bacteria are formed when these undergo conjugation with F- (fertility factor) cells. F factor incorporates at a particular location in the genome of bacteria and while the order of donating genes is such that the gene preceding the F factor appears first and the sequence continues.
The number of genes transferred depends on the duration of conjugation. The longer the duration is, the more the genes are transferred. Also, different genes are transferred during different conjugations.
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A series of Hfr strains that have genotype m+ n+ o+ p+ q+ r+ are mixed with an F− strain that has genotype m− n− o− p− q− r−. Conjugation is interrupted at regular intervals and the order of the appearance of genes from the Hfr strain is determined in the recipient cells. The order of gene transfer for each Hfr strain is What is the order of genes on the circular bacterial chromosome? For each Hfr strain, give the location of the F factor in the chromosome and its polarity. Hfr5 m+ q+ p+ n+ r+ o+ Hfr4 n+ r+ o+ m+ q+ p+ Hfr1 o+ m+ q+ p+ n+ r+ Hfr9 q+ m+ o+ r+ n+ p+
Determine the map distances between the genes.
In E. coli, the gene bioD+ encodes an enzyme involved in biotin synthesis, and galK+ encodes an enzyme involved in galactose utilization. An E. coli strain that contained wild-type versions of both genes was infected with P1 phage, and then a P1 lysate was obtained. This lysate was used totransduce (infect) a strain that was bioD− and galK−. The cellswere plated on a medium containing galactose as the sole carbonsource for growth to select for transduction of the galK+ gene.This medium also was supplemented with biotin. The resultingcolonies were then restreaked on a medium that lacked biotin tosee if the bioD+ gene had been cotransduced. The following resultswere obtained:What information do you know based onthe question and your understanding of the topic?
Chapter 17 Solutions
Biology: The Dynamic Science (MindTap Course List)
Ch. 17.1 - What are the properties of F+, F-, and Hfr cells...Ch. 17.1 - Prob. 2SBCh. 17.2 - Prob. 1SBCh. 17.2 - How does viral infection of an animal cell and a...Ch. 17.2 - Prob. 3SBCh. 17.3 - Prob. 1SBCh. 17 - Prob. 1TYKCh. 17 - Prob. 2TYKCh. 17 - Which of the following is not correct for...Ch. 17 - Prob. 4TYK
Ch. 17 - Prob. 5TYKCh. 17 - Prob. 6TYKCh. 17 - When a virus enters the lysogenic stage: a. the...Ch. 17 - An infectious material is isolated from a nerve...Ch. 17 - Prob. 9TYKCh. 17 - Prob. 10TYKCh. 17 - Prob. 11TYKCh. 17 - Discuss Concepts As a control for their...Ch. 17 - Prob. 13TYKCh. 17 - Prob. 14TYKCh. 17 - Prob. 15TYKCh. 17 - Prob. 1ITD
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