Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Chapter 17, Problem 14TYK
Summary Introduction
To review:
The experiment that can confirm the presence of a wild type bio+ allele in the plasmid of chromosomal DNA (deoxyribonucleic acid).
Introduction:
Minimal media is the media that only provides minimum requirement that is only inorganic salts and no amino acids. The colonies whose genome is complete, that is, they have genes for all requirements grows on the minimal media while others do not.
Electroporation is the technique in which electric pulse is given to the culture leading the opening of the transient pores in the cells that can allow the entry of the DNA from the surrounding.
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You have set up a recombinant DNA experiment using the plasmid PBR322 as the vector (see plasmid below). You use the BamHI restriction site on the plasmid to insert the target DNA. The
plasmid is then used to transform E.coli colls Is the following statement True or False?
Growth of the transformed cells on agar containing both ampicillin and tetracycline will eliminate any cells that do not contain a plasmid.
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PBR322
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True
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you have transformed E.coli cells with a plasmid containing the gene for the enzyme beta-lactamase you have used 3µl of a stock solution of 2.5ng/µl DNA. You transformed 150/µl of competent cells, using the calcium chloride method. you have added 600µl of luria broth to the cells and then plated out 200µl on triplicate plates. upon counting the colonies, you got the following results:
plate 1: 120 cfu
plate 2: 35 cfu
plate 3: 95 cfu
please answer the following question:
1. what new characteristic will the cells have ater transformation?
2. how are you going to test for the new characteristic?
3. calculate the transformation efficiency of the experiment?
You have just carried out a transformation using a plasmid (possessing a
Amp-resistance gene) that was 0.001 ug/ul in concentration. You added
10 ul of this plasmid to 100 ul of a bacterial cell suspension. Then
you
added 250 ul of LB following heat shock. After plating 200 ul of this cell
suspension with LB onto a LBA plate, you observe 8 colonies on this
plate. What is the transformation efficiency of this plasmid (in
colonies/ug of plasmid) based on these results?
about 1400
O about 80O
about 4000
O about 400O
O about 8000
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Chapter 17 Solutions
Biology: The Dynamic Science (MindTap Course List)
Ch. 17.1 - What are the properties of F+, F-, and Hfr cells...Ch. 17.1 - Prob. 2SBCh. 17.2 - Prob. 1SBCh. 17.2 - How does viral infection of an animal cell and a...Ch. 17.2 - Prob. 3SBCh. 17.3 - Prob. 1SBCh. 17 - Prob. 1TYKCh. 17 - Prob. 2TYKCh. 17 - Which of the following is not correct for...Ch. 17 - Prob. 4TYK
Ch. 17 - Prob. 5TYKCh. 17 - Prob. 6TYKCh. 17 - When a virus enters the lysogenic stage: a. the...Ch. 17 - An infectious material is isolated from a nerve...Ch. 17 - Prob. 9TYKCh. 17 - Prob. 10TYKCh. 17 - Prob. 11TYKCh. 17 - Discuss Concepts As a control for their...Ch. 17 - Prob. 13TYKCh. 17 - Prob. 14TYKCh. 17 - Prob. 15TYKCh. 17 - Prob. 1ITD
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