CONNECT ACCESS CARD FOR ANATOMY AND PHYSIOLOGY
8th Edition
ISBN: 9781259880193
Author: SALADIN
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Textbook Question
Chapter 16.5, Problem 8AYLO
General structure of the retina: its two points of attachment to the wall of the eye: and the locations, structure, and functional significance of the optic disc, optic nerve, macula lutea. and fovea centralis
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 16 Solutions
CONNECT ACCESS CARD FOR ANATOMY AND PHYSIOLOGY
Ch. 16.1 - Not every sensory receptor is a sense organ....Ch. 16.1 - What does it mean to say sense organs are...Ch. 16.1 - Prob. 3BYGOCh. 16.1 - What is meant by the modality of a stimulus? Give...Ch. 16.1 - Three schemes of receptor classification were...Ch. 16.1 - Nociceptors are tonic rather than phasic...Ch. 16.1 - The definition of receptor and the range of...Ch. 16.1 - Prob. 2AYLOCh. 16.1 - Prob. 3AYLOCh. 16.1 - Prob. 4AYLO
Ch. 16.1 - Prob. 5AYLOCh. 16.1 - Prob. 6AYLOCh. 16.1 - Prob. 7AYLOCh. 16.2 - What stimulus modalities are detected by free...Ch. 16.2 - Prob. 8BYGOCh. 16.2 - Where do most second-order somatosensory neurons...Ch. 16.2 - Explain the phenomenon of referred pain in terms...Ch. 16.2 - Explain the roles of bradykinin, substance P, and...Ch. 16.2 - Three Types of unencapsulated sensory nerve...Ch. 16.2 - Six types of encapsulated nerve endings and how...Ch. 16.2 - The main routes for somatosensory signals from the...Ch. 16.2 - Definitions of pain and nociceptorCh. 16.2 - Differences between fast pain and slow painCh. 16.2 - Differences between somatic and visceral pain, and...Ch. 16.2 - Several chemicals released by injured tissues that...Ch. 16.2 - The general three-neuron pathway typically taken...Ch. 16.2 - Pain pathways from receptors in the head to the...Ch. 16.2 - Pain pathways from the lower body to the cerebral...Ch. 16.2 - Prob. 11AYLOCh. 16.2 - Referred pain and its anatomical basisCh. 16.2 - Prob. 13AYLOCh. 16.2 - Prob. 14AYLOCh. 16.3 - Prob. 12BYGOCh. 16.3 - Prob. 13BYGOCh. 16.3 - Prob. 14BYGOCh. 16.3 - Prob. 15BYGOCh. 16.3 - Prob. 16BYGOCh. 16.3 - Prob. 1AYLOCh. 16.3 - Types, locations, and functions of lingual...Ch. 16.3 - Five primary taste sensations, and sensations...Ch. 16.3 - Prob. 4AYLOCh. 16.3 - Prob. 5AYLOCh. 16.3 - Prob. 6AYLOCh. 16.3 - How odor molecules excite olfactory cellsCh. 16.3 - Prob. 8AYLOCh. 16.3 - Prob. 9AYLOCh. 16.3 - Prob. 10AYLOCh. 16.4 - Prob. 17BYGOCh. 16.4 - Prob. 18BYGOCh. 16.4 - Prob. 19BYGOCh. 16.4 - Prob. 20BYGOCh. 16.4 - Prob. 21BYGOCh. 16.4 - Prob. 22BYGOCh. 16.4 - Prob. 1AYLOCh. 16.4 - Prob. 2AYLOCh. 16.4 - Prob. 3AYLOCh. 16.4 - Prob. 4AYLOCh. 16.4 - Prob. 5AYLOCh. 16.4 - Prob. 6AYLOCh. 16.4 - The bony and membranous labyrinths of the inner...Ch. 16.4 - Prob. 8AYLOCh. 16.4 - Prob. 9AYLOCh. 16.4 - Prob. 10AYLOCh. 16.4 - Prob. 11AYLOCh. 16.4 - Prob. 12AYLOCh. 16.4 - Prob. 13AYLOCh. 16.4 - Prob. 14AYLOCh. 16.4 - The pathway from cochlear nerve to auditory...Ch. 16.4 - Prob. 16AYLOCh. 16.4 - Prob. 17AYLOCh. 16.4 - Prob. 18AYLOCh. 16.4 - Prob. 19AYLOCh. 16.4 - Prob. 20AYLOCh. 16.4 - Prob. 21AYLOCh. 16.4 - The path taken by signals in the vestibular nerve...Ch. 16.4 - Prob. 23AYLOCh. 16.5 - Why can't we see wavelengths of 350 nm or 750 nm?Ch. 16.5 - Prob. 24BYGOCh. 16.5 - Prob. 25BYGOCh. 16.5 - Prob. 26BYGOCh. 16.5 - Prob. 27BYGOCh. 16.5 - Prob. 1AYLOCh. 16.5 - Six extrinsic eye muscles, their anatomy, the eye...Ch. 16.5 - Prob. 3AYLOCh. 16.5 - Prob. 4AYLOCh. 16.5 - Prob. 5AYLOCh. 16.5 - Prob. 6AYLOCh. 16.5 - Prob. 7AYLOCh. 16.5 - General structure of the retina: its two points of...Ch. 16.5 - Prob. 9AYLOCh. 16.5 - Prob. 10AYLOCh. 16.5 - Principles of refraction; points at which...Ch. 16.5 - The difference between enmetropia and lite near...Ch. 16.5 - Histological layers and cell types of the retina....Ch. 16.5 - The structures of rods and cones; where visual...Ch. 16.5 - Differences in rod and cone functionCh. 16.5 - Prob. 16AYLOCh. 16.5 - Prob. 17AYLOCh. 16.5 - Why a single retinal receptor system cannot...Ch. 16.5 - Difference between S, M, and L cones; how neural...Ch. 16.5 - Prob. 20AYLOCh. 16.5 - Prob. 21AYLOCh. 16.5 - Hemidecussation, where it occurs, and how it...Ch. 16 - Hot and cold stimuli are detected by a. free nerve...Ch. 16 - _____is a neurotransmitter that transmits pain...Ch. 16 - Prob. 3TYRCh. 16 - Prob. 4TYRCh. 16 - Prob. 5TYRCh. 16 - Prob. 6TYRCh. 16 - Prob. 7TYRCh. 16 - Prob. 8TYRCh. 16 - The retina receives its oxygen supply from a. the...Ch. 16 - Prob. 10TYRCh. 16 - The most finely detailed vision occurs when an...Ch. 16 - Prob. 12TYRCh. 16 - Prob. 13TYRCh. 16 - Prob. 14TYRCh. 16 - Three rows of________in the cochlea have V-shaped...Ch. 16 - The ______ is a tiny bone that vibrates in the...Ch. 16 - The _____ of the midbrain receives auditory input...Ch. 16 - Prob. 18TYRCh. 16 - Olfactory neurons synapse with mitral cells and...Ch. 16 - In the phenomenon of ______, pain from the viscera...Ch. 16 - bin-Ch. 16 - decuss-Ch. 16 - hemi-Ch. 16 - lacrimo-Ch. 16 - litho-Ch. 16 - maculo-Ch. 16 - noci-Ch. 16 - scoto-Ch. 16 - -sepsisCh. 16 - Prob. 10BYMVCh. 16 - The sensory (afferent) nerve fibers for touch end...Ch. 16 - Prob. 2WWTSCh. 16 - Things we see with the left eye are perceived only...Ch. 16 - Prob. 4WWTSCh. 16 - The vitreous body occupies the posterior chamber...Ch. 16 - Prob. 6WWTSCh. 16 - Prob. 7WWTSCh. 16 - Prob. 8WWTSCh. 16 - Prob. 9WWTSCh. 16 - Prob. 10WWTSCh. 16 - The principle of neural convergence is explained...Ch. 16 - What type of cutaneous receptor enables you to...Ch. 16 - Prob. 3TYCCh. 16 - Prob. 4TYCCh. 16 - Prob. 5TYC
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