Biology: The Dynamic Science (MindTap Course List)
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Chapter 14.1, Problem 1SB
Summary Introduction

To review:

The differences between results of Hershey and Chase’s original experiment and in new experiment in which 35S (radioactive isotope of sulfur) labeled both protein and DNA, whereas 32P (radioactive isotope of phosphorous) labeled only DNA.

Introduction:

Living beings transfer characters from one generation to another and the molecule responsible for transferring these characters is called genetic material. In the first half of the 20th century, scientists wondered which molecule of cell contains the genetic material; different scientists performed different experiments to find it. Hershey and Chase’s experiment gave evidence that DNA is the genetic material, after the experiments of Griffith (a substance transformed the bacteria pneumonia genetically), and the experiments of Avery and his coworkers (DNA is the genetic material that transformed nonvirulent bacteria to virulent bacteria).

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a) Explain the effect of the guanine:cytosine ratio on melting temperature of DNA. b) The Hershey-Chase experiment proved that DNA is the genetic material and not protein. Explain in detatil how this experiment was conducted.
4b
Consider the experiment conducted by Meselson and Stahl in which they used 14N and 15N in cultures of E. coli and equilibrium density gradient centrifugation. Draw pictures to represent the bands produced by bacterial DNA in the centrifuge tube before the switch to medium containing 14N and after one, two, and three rounds of replication in that medium. Use separate sets of drawings to show the bands that would appear if replication were (a) semiconservative; (b) conservative; (c) dispersive.
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