To review:
The differences between results of Hershey and Chase’s original experiment and in new experiment in which 35S (radioactive isotope of sulfur) labeled both protein and DNA, whereas 32P (radioactive isotope of phosphorous) labeled only DNA.
Introduction:
Living beings transfer characters from one generation to another and the molecule responsible for transferring these characters is called genetic material. In the first half of the 20th century, scientists wondered which molecule of cell contains the genetic material; different scientists performed different experiments to find it. Hershey and Chase’s experiment gave evidence that DNA is the genetic material, after the experiments of Griffith (a substance transformed the bacteria pneumonia genetically), and the experiments of Avery and his coworkers (DNA is the genetic material that transformed nonvirulent bacteria to virulent bacteria).
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Chapter 14 Solutions
Biology: The Dynamic Science (MindTap Course List)
- Provide a brief summary of the Sanger sequencing method.arrow_forwardThe DNA chromosome in E. coli contains approximately 4 million base pairs. The average gene contains about 1500 base pairs. Use this information to calculate the following (show all work ): a) The length in meters of this chromosome. b) The approximate number of genes in the chromosome (assuming no wasted DNA).arrow_forwardWhen proteins are separated using native gel electrophoresis, size, shape, and charge control their rate of migration on the gel. Why does DNA separate based on size, and why do we not worry much about shape or charge?arrow_forward
- Can you please help with 1c please picture with 1 graph is for question 1a) picture with 4 graphs is for question 1b) 1a) E. coli DNA and binturong DNA are both 50% G-C. If you randomly shear E. coli DNA into 1000 bp fragments and put it through density gradient equilibrium centrifugation, you will find that all the DNA bands at the same place in the gradient, and if you graph the distribution of DNA fragments in the gradient you will get a single peak (see below). If you perform the same experiment with binturong DNA, you will find that a small fraction of the DNA fragments band separately in the gradient (at a different density) and give rise to a small "satellite" peak on a graph of the distribution of DNA fragments in the gradient (see below). Why do these two DNA samples give different results, when they're both 50% G-C? 1b) If you denatured the random 1000 bp fragments of binturong DNA that you produced in question 1a by heating them to 95ºC, and then cooled them down to 60ºC…arrow_forwardState the properties of the Watson-Crick model of DNA in the following categories: a) number of polynucleotide chains b) polarity (strand direction running same or opposite c) bases on interior or exterior of molecule d) sugar/phosphate on interior or exterior of molecule e) which bases pair with which f) right- or left-handed helixarrow_forwardDiagram and explain how APEX probes can be used to determine that an individual is CC (homozygous) for a specific G/C SNV. Recall that the genotype of an SNV is identified from the strand shown in NCBI. What color fluorescence will be observed? Also, explain why a left apex probe cannot be used for this SNV. The SNV sequence, on the strand shown in NCBI, and a few nucleotides adjacent to the SNV are below: 5'-------TGT(G/C)CAG------3'arrow_forward
- Using the first and second base key below, predict the DNA sequence given by the SOLID color sequence. For the key G = green, R = red, Y = yellow, and B = blue. Note that the first base of the sequence is already given ("A"). Give the remaining 8 bases for this sequence. A First base A CCT Second base A CGT BGY R GBRY RBG R Y (G) B Y G)(R) GB )( R )( Y ) ( G) Barrow_forwardExplain the principles behind DNA isolation using silica membrane spin column from Qiagen. 2 After DNA isolation using Qiagen extraction kit, the final flow through was subjected to spectrophotometric measurements. Explain the situation if the absorbances were found to be: i) The absorbance at 260 nm is higher than absorbance at 280 nm? ii) The absorbance at 230 is higher than absorbance at 260 nm?arrow_forwardLet’s assume the linker region of DNA averages 54 bp in length. How many molecules of H2A would you expect to find in a DNA sample that is 46,000 bp in length?arrow_forward
- Can you please help with 1f please picture with 1 graph is for question 1a) picture with 4 graphs is for question 1b) 1a) E. coli DNA and binturong DNA are both 50% G-C. If you randomly shear E. coli DNA into 1000 bp fragments and put it through density gradient equilibrium centrifugation, you will find that all the DNA bands at the same place in the gradient, and if you graph the distribution of DNA fragments in the gradient you will get a single peak (see below). If you perform the same experiment with binturong DNA, you will find that a small fraction of the DNA fragments band separately in the gradient (at a different density) and give rise to a small "satellite" peak on a graph of the distribution of DNA fragments in the gradient (see below). Why do these two DNA samples give different results, when they're both 50% G-C? 1b) If you denatured the random 1000 bp fragments of binturong DNA that you produced in question 1a by heating them to 95ºC, and then cooled them down to 60ºC and…arrow_forwardA purified recombinant protein is analyzed for molecular weight by SDS-PAGE at pH 8.5. From the protein sequence deduced from the gene that was expressed in bacteria, the protein is expected to have a molecular weight of 44,000. However, the molecular weight of the protein is found by SDS-PAGE to be 52,000. Explain the reason or reasons for this difference in molecular weight. What calculation could you make to help explain this discrepancy?arrow_forward1a) When performing classical Sanger or "dideoxy" sequencing, you set up 4 parallel reactions per template to be sequenced from a specific primer, with each of the four reactions containing a different dideoxynucleotide, and then the four reactions were run in a separate, adjacent lanes on a gel. Why couldn't you combine all 4 dideoxynucleotides with the primer and the template and do the whole reaction in one tube, and then run the set of fragments produced by the reaction mixture on a single lane in an acrylamide gel?arrow_forward
- Biology: The Dynamic Science (MindTap Course List)BiologyISBN:9781305389892Author:Peter J. Russell, Paul E. Hertz, Beverly McMillanPublisher:Cengage LearningBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax